A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish

The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of Eubothrium crassum and Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for E. crassum, a...

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Veröffentlicht in:International journal for parasitology 2001, Vol.31 (1), p.93-96
Hauptverfasser: Král’ová, I., Hanzelová, V., Scholz, T., Gerdeaux, D., Špakulová, M.
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Sprache:eng
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Zusammenfassung:The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of Eubothrium crassum and Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for E. crassum, and 1117 bp and 50.5% for E. salvelini, respectively. Sequence alignment and comparison of both taxa showed 97.9% similarity. Within the ITS-1 and ITS-2 of both species, the restriction enzymes MspI and Bsh1236I possessed different cleavage sites and could be employed for rapid differentiation of both species of Eubothrium by PCR-RFLP.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(00)00147-8