Assembly of Marek's disease virus (MDV) capsids using recombinant baculoviruses expressing MDV capsid proteins

Laboratoire de Virologie et Barrière d'Espèces, UR086, INRA, Centre de Recherche de Tours, 37380 Nouzilly, France Correspondence D. Rasschaert rasschae{at}tours.inra.fr The genes UL18 , UL19 , UL26 , UL26.5 , UL35 and UL38 of Marek's disease virus 1 (MDV-1) strain RB1B, encoding the homologues of herpes simplex virus type 1 (HSV-1) capsid proteins VP23, VP5, VP21–VP24, preVP22a, VP26 and VP19C, were identified and sequenced. Recombinant baculoviruses were used to express the six capsid genes in insect cells. Coexpression of the six genes or of UL18 , UL19 , UL26.5 and UL38 in insect cells resulted in the formation of capsids with a large core. In addition, electron microscopy of thin sections clearly revealed the presence of large numbers of small spherical particles. Experimental coinfection demonstrated that these small particles were associated with production of the preVP22a protein.