Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity

The purpose of this study was to evaluate the effects of phytic acid (IP6) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5mM phytic acid decreases cell viability and tr...

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Veröffentlicht in:	Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2012-05, Vol.64 (4), p.345-347
Hauptverfasser:	Pacheco, Graziela Drociunas, Silva, Caio Abércio da, Pinton, Philippe, Oswald, Isabelle P., Bracarense, Ana Paula Frederico Rodrigues Loureiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Membrane Permeability - drug effects Cell Membrane Permeability - physiology Cell Survival - drug effects Cells, Cultured Electrophysiological Phenomena - drug effects Inositol Intestinal cell Intestinal Mucosa - drug effects Intestinal Mucosa - pathology Intestinal Mucosa - physiology Intestine, Small - drug effects Intestine, Small - pathology Intestine, Small - physiology Investigative techniques, diagnostic techniques (general aspects) IP6 Life Sciences Medical sciences Mycotoxin Patch-Clamp Techniques Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phytic Acid - pharmacology Swine Toxicity Trichothecenes - toxicity
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Zusammenfassung:	The purpose of this study was to evaluate the effects of phytic acid (IP6) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed that treatment with 0.5mM or 1.0mM phytic acid restores the decrease in TEER caused by 25μM DON. In conclusion this study demonstrates that phytic acid decreased the negative effects of deoxynivalenol on the membrane integrity of the IPEC-1 intestinal epithelial cell line.
ISSN:	0940-2993 1618-1433
DOI:	10.1016/j.etp.2010.09.008