Anthocyanin identification and composition of wild Vitis spp. accessions by using LC–MS and LC–NMR

The skin berry composition and concentration of anthocyanins in 4 different species were analyzed: V. vinifera, V. amurensis, V. cinerea and V. X champinii. LC–MS and LC–NMR were used to separate and identify the structure of anthocyanins present in these species. In particular, newly reported cis isomers of p-coumaric-derivatives were identified. [Display omitted] ► We analyzed the composition and concentration of anthocyanins of 4 Vitis species. ► We used LC–MS and LC–NMR to separate and identify the structure of anthocyanins. ► We identified 33 different anthocyanins. ► We identified newly reported cis isomers of p-coumaric-derivatives. The composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: Vitis vinifera, Vitis amurensis, Vitis cinerea and Vitis X champinii. High-performance liquid chromatography coupled to mass spectrometry (LC–MS) and NMR spectroscopy (LC–NMR) were used to separate and identify the structure of anthocyanins present in these species. Combination of LC–MS and LC–NMR data resulted in the identification of 33 anthocyanins. In particular, newly reported cis isomers of p-coumaric-derivatives were identified (petunidin-, peonidin- and malvidin-3-(6-p-coumaroyl)-5-diglucoside). In V. cinerea and V. vinifera, anthocyanins were monoglucoside derivatives whereas in V. amurensis and V. X champinii, both mono- and diglucoside derivatives were identified. Malvidin-, delphinidin- and petunidin-derivatives were, respectively, the most abundant components in V. cinerea and V. vinifera, V. amurensis and V. X champinii.