Molecular genetic diversity of Gongylonema neoplasticum (Fibiger & Ditlevsen, 1914) (Spirurida: Gongylonematidae) from rodents in Southeast Asia
More than a dozen Gongylonema spp. (Spirurida: Spiruroidea: Gongylonematidae) have been described from a variety of rodent hosts worldwide. Gongylonema neoplasticum (Fibiger & Ditlevsen, 1914), which dwells in the gastric mucosa of rats such as Rattus norvegicus (Berkenhout) and Rattus rattus (L...
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Veröffentlicht in: | Systematic parasitology 2018-03, Vol.95 (2-3), p.235-247 |
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Zusammenfassung: | More than a dozen
Gongylonema
spp. (Spirurida: Spiruroidea: Gongylonematidae) have been described from a variety of rodent hosts worldwide.
Gongylonema neoplasticum
(Fibiger & Ditlevsen, 1914), which dwells in the gastric mucosa of rats such as
Rattus norvegicus
(Berkenhout) and
Rattus rattus
(Linnaeus), is currently regarded as a cosmopolitan nematode in accordance with global dispersion of its definitive hosts beyond Asia. To facilitate the reliable specific differentiation of local rodent
Gongylonema
spp. from the cosmopolitan congener, the genetic characterisation of
G. neoplasticum
from Asian
Rattus
spp. in the original endemic area should be considered since the morphological identification of
Gongylonema
spp. is often difficult due to variations of critical phenotypical characters, e.g. spicule lengths and numbers of caudal papillae. In the present study, morphologically identified
G. neoplasticum
from 114 rats of seven species from Southeast Asia were selected from archived survey materials from almost 4,500 rodents: Thailand (58 rats), Cambodia (52 rats), Laos (three rats) and Philippines (one rat). In addition, several specimens from four rats in Indonesia were used in the study. Nucleotide sequences of the ribosomal RNA gene (rDNA) (5,649 bp) and the cytochrome
c
oxidase subunit 1 gene (
cox
1) (818 bp) were characterised. The rDNA showed little nucleotide variation, including the internal transcribed spacer (ITS) regions. The
cox
1 showed 24 haplotypes, with up to 15 (1.83%) nucleotide substitutions regardless of parasite origin. Considering that
Rattus
spp. have been shown to originate from the southern region of Asia and
G. neoplasticum
is their endogenous parasite, it is reasonable to propose that the present study covers a wide spectrum of the genetic diversity of
G. neoplasticum
, useful for both the molecular genetic speculation of the species and the molecular genetic differentiation of other local rodent
Gongylonema
spp. from the cosmopolitan congener. |
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ISSN: | 0165-5752 1573-5192 |
DOI: | 10.1007/s11230-018-9778-0 |