Synthesis and mannose receptor-Mediated uptake of clustered glycomimetics by human dendritic cells: effect of charge

Effect of charge and shape of multivalent lysine-based cluster glycomimetics on their mannose receptor-mediated uptake by human dendritic cells has been evaluated: The capture is strongly affected by the shape of the ligands. The effect of charge is less pronounced although positive charges on the ligands seem to favor non-specific endocytosis capture. A set of fluorescein-labeled quinoylated or shikimoylated clusters is reported. Their specific capture by human dendritic cells was assessed on the basis of their overall charge and shape using flow cytometry analysis.