Measurement of isotopic enrichments in 13C-labelled molecules by 1D selective Zero-Quantum Filtered TOCSY NMR experiments
A NMR method based on the combination of selective pulses with Zero-Quantum Filtered TOCSY magnetization transfer was used to measure the specific 13C-enrichment of labelled molecules. The analysis of a labelled lysine sample showed that DANTE-Z schemes give accurate measurements; meanwhile, DPFGSE...
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| Veröffentlicht in: | Comptes rendus. Chimie 2008-04, Vol.11 (4), p.480-485 |
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| creator | Nicolas, Cécile Becker, Judith Sanchou, Laurent Letisse, Fabien Wittmann, Christoph Portais, Jean-Charles Massou, Stéphane |
| description | A NMR method based on the combination of selective pulses with Zero-Quantum Filtered TOCSY magnetization transfer was used to measure the specific
13C-enrichment of labelled molecules. The analysis of a labelled lysine sample showed that DANTE-Z schemes give accurate measurements; meanwhile, DPFGSE schemes resulted in the underestimation of
13C-enrichments. The former method can be applied to overcome signal overlap problems that are often encountered in the analysis of labelled molecules.
Une méthode RMN basée sur l'association d'impulsions sélectives et de transfert d'aimantation de type TOCSY avec des filtres à zéro quantum a été utilisée pour mesurer l'enrichissement spécifique en
13C de molécules marquées. L'analyse d'une solution de lysine marquée a permis de montrer que des modules de type DANTE-Z donnent des mesures fiables, contrairement à l'utilisation de modules DPFGSE qui conduisent à une sous-estimation de l'enrichissement. La première méthode permet de s'affranchir de problèmes de superposition de signaux fréquemment rencontrés dans l'analyse de molécules marquées. |
| doi_str_mv | 10.1016/j.crci.2007.06.018 |
| format | Article |
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13C-enrichment of labelled molecules. The analysis of a labelled lysine sample showed that DANTE-Z schemes give accurate measurements; meanwhile, DPFGSE schemes resulted in the underestimation of
13C-enrichments. The former method can be applied to overcome signal overlap problems that are often encountered in the analysis of labelled molecules.
Une méthode RMN basée sur l'association d'impulsions sélectives et de transfert d'aimantation de type TOCSY avec des filtres à zéro quantum a été utilisée pour mesurer l'enrichissement spécifique en
13C de molécules marquées. L'analyse d'une solution de lysine marquée a permis de montrer que des modules de type DANTE-Z donnent des mesures fiables, contrairement à l'utilisation de modules DPFGSE qui conduisent à une sous-estimation de l'enrichissement. La première méthode permet de s'affranchir de problèmes de superposition de signaux fréquemment rencontrés dans l'analyse de molécules marquées.</description><identifier>ISSN: 1631-0748</identifier><identifier>ISSN: 1878-1543</identifier><identifier>EISSN: 1878-1543</identifier><identifier>DOI: 10.1016/j.crci.2007.06.018</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; DANTE-Z ; DPFGSE ; Enrichissement spécifique en 13C ; Flux métaboliques ; Fundamental and applied biological sciences. Psychology ; Life Sciences ; Metabolic fluxes ; NMR ; RMN ; Specific 13C-enrichment ; ZQF-TOCSY</subject><ispartof>Comptes rendus. Chimie, 2008-04, Vol.11 (4), p.480-485</ispartof><rights>2007 Académie des sciences</rights><rights>2008 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2538-51effbed41dcf7ee36dd15af18ef612d99a35a60d1154ce103aaed219a3245fe3</citedby><cites>FETCH-LOGICAL-c2538-51effbed41dcf7ee36dd15af18ef612d99a35a60d1154ce103aaed219a3245fe3</cites><orcidid>0000-0002-1490-0152 ; 0000-0002-3480-0933</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.crci.2007.06.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,309,310,314,780,784,789,790,885,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20368588$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://insa-toulouse.hal.science/hal-02183131$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Nicolas, Cécile</creatorcontrib><creatorcontrib>Becker, Judith</creatorcontrib><creatorcontrib>Sanchou, Laurent</creatorcontrib><creatorcontrib>Letisse, Fabien</creatorcontrib><creatorcontrib>Wittmann, Christoph</creatorcontrib><creatorcontrib>Portais, Jean-Charles</creatorcontrib><creatorcontrib>Massou, Stéphane</creatorcontrib><title>Measurement of isotopic enrichments in 13C-labelled molecules by 1D selective Zero-Quantum Filtered TOCSY NMR experiments</title><title>Comptes rendus. Chimie</title><description>A NMR method based on the combination of selective pulses with Zero-Quantum Filtered TOCSY magnetization transfer was used to measure the specific
13C-enrichment of labelled molecules. The analysis of a labelled lysine sample showed that DANTE-Z schemes give accurate measurements; meanwhile, DPFGSE schemes resulted in the underestimation of
13C-enrichments. The former method can be applied to overcome signal overlap problems that are often encountered in the analysis of labelled molecules.
Une méthode RMN basée sur l'association d'impulsions sélectives et de transfert d'aimantation de type TOCSY avec des filtres à zéro quantum a été utilisée pour mesurer l'enrichissement spécifique en
13C de molécules marquées. L'analyse d'une solution de lysine marquée a permis de montrer que des modules de type DANTE-Z donnent des mesures fiables, contrairement à l'utilisation de modules DPFGSE qui conduisent à une sous-estimation de l'enrichissement. La première méthode permet de s'affranchir de problèmes de superposition de signaux fréquemment rencontrés dans l'analyse de molécules marquées.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>DANTE-Z</subject><subject>DPFGSE</subject><subject>Enrichissement spécifique en 13C</subject><subject>Flux métaboliques</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences</subject><subject>Metabolic fluxes</subject><subject>NMR</subject><subject>RMN</subject><subject>Specific 13C-enrichment</subject><subject>ZQF-TOCSY</subject><issn>1631-0748</issn><issn>1878-1543</issn><issn>1878-1543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kD9PwzAQxSMEElD4AkxeGBgSfHHjuhJLVSggFRD_Blgs1z4LV25S2WlFvz0ORR2Z7u7p_e50L8vOgBZAgV_OCx20K0pKBwXlBQWxlx2BGIgcqj7bTz1nkNNBXxxmxzHOaYKGFT_KNg-o4irgAuuWNJa42LTN0mmCdXD6q5MjcTUBNs69mqH3aMii8ahXHiOZbQhck4hpbt0aySeGJn9eqbpdLcjE-RZD8r89jV8_yOPDC8HvJQb3u_UkO7DKRzz9q73sfXLzNr7Lp0-39-PRNNdlxUReAVo7Q9MHo-0AkXFjoFIWBFoOpRkOFasUpwbSpxqBMqXQlJDksl9ZZL3sYrv3S3m5TMdV2MhGOXk3mspOoyUIBgzWkLzl1qtDE2NAuwOAyi5oOZdd0LILWlIuU9AJOt9CSxW18jaoWru4I0vKuKhE57va-jB9u3YYZNQOa43GhRSfNI3778wPHLCUsg</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Nicolas, Cécile</creator><creator>Becker, Judith</creator><creator>Sanchou, Laurent</creator><creator>Letisse, Fabien</creator><creator>Wittmann, Christoph</creator><creator>Portais, Jean-Charles</creator><creator>Massou, Stéphane</creator><general>Elsevier SAS</general><general>Elsevier</general><general>Académie des sciences (Paris)</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-1490-0152</orcidid><orcidid>https://orcid.org/0000-0002-3480-0933</orcidid></search><sort><creationdate>200804</creationdate><title>Measurement of isotopic enrichments in 13C-labelled molecules by 1D selective Zero-Quantum Filtered TOCSY NMR experiments</title><author>Nicolas, Cécile ; Becker, Judith ; Sanchou, Laurent ; Letisse, Fabien ; Wittmann, Christoph ; Portais, Jean-Charles ; Massou, Stéphane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2538-51effbed41dcf7ee36dd15af18ef612d99a35a60d1154ce103aaed219a3245fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>DANTE-Z</topic><topic>DPFGSE</topic><topic>Enrichissement spécifique en 13C</topic><topic>Flux métaboliques</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Life Sciences</topic><topic>Metabolic fluxes</topic><topic>NMR</topic><topic>RMN</topic><topic>Specific 13C-enrichment</topic><topic>ZQF-TOCSY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nicolas, Cécile</creatorcontrib><creatorcontrib>Becker, Judith</creatorcontrib><creatorcontrib>Sanchou, Laurent</creatorcontrib><creatorcontrib>Letisse, Fabien</creatorcontrib><creatorcontrib>Wittmann, Christoph</creatorcontrib><creatorcontrib>Portais, Jean-Charles</creatorcontrib><creatorcontrib>Massou, Stéphane</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Comptes rendus. Chimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nicolas, Cécile</au><au>Becker, Judith</au><au>Sanchou, Laurent</au><au>Letisse, Fabien</au><au>Wittmann, Christoph</au><au>Portais, Jean-Charles</au><au>Massou, Stéphane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of isotopic enrichments in 13C-labelled molecules by 1D selective Zero-Quantum Filtered TOCSY NMR experiments</atitle><jtitle>Comptes rendus. Chimie</jtitle><date>2008-04</date><risdate>2008</risdate><volume>11</volume><issue>4</issue><spage>480</spage><epage>485</epage><pages>480-485</pages><issn>1631-0748</issn><issn>1878-1543</issn><eissn>1878-1543</eissn><abstract>A NMR method based on the combination of selective pulses with Zero-Quantum Filtered TOCSY magnetization transfer was used to measure the specific
13C-enrichment of labelled molecules. The analysis of a labelled lysine sample showed that DANTE-Z schemes give accurate measurements; meanwhile, DPFGSE schemes resulted in the underestimation of
13C-enrichments. The former method can be applied to overcome signal overlap problems that are often encountered in the analysis of labelled molecules.
Une méthode RMN basée sur l'association d'impulsions sélectives et de transfert d'aimantation de type TOCSY avec des filtres à zéro quantum a été utilisée pour mesurer l'enrichissement spécifique en
13C de molécules marquées. L'analyse d'une solution de lysine marquée a permis de montrer que des modules de type DANTE-Z donnent des mesures fiables, contrairement à l'utilisation de modules DPFGSE qui conduisent à une sous-estimation de l'enrichissement. La première méthode permet de s'affranchir de problèmes de superposition de signaux fréquemment rencontrés dans l'analyse de molécules marquées.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><doi>10.1016/j.crci.2007.06.018</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-1490-0152</orcidid><orcidid>https://orcid.org/0000-0002-3480-0933</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences DANTE-Z DPFGSE Enrichissement spécifique en 13C Flux métaboliques Fundamental and applied biological sciences. Psychology Life Sciences Metabolic fluxes NMR RMN Specific 13C-enrichment ZQF-TOCSY |
| title | Measurement of isotopic enrichments in 13C-labelled molecules by 1D selective Zero-Quantum Filtered TOCSY NMR experiments |
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