Pro-oxidant effects of nano-TiO 2 on Chlamydomonas reinhardtii during short-term exposure

This study sheds light on the short-term dynamics of pro-oxidant processes related to the exposure of C. reinhardtii microalgae to nano-TiO 2 using (a) conventional fluorescence probes for cellular pro-oxidant process and (b) a recently developed cytochrome c biosensor for the continuous quantification of extracellular H 2 O 2 . The main aims are to investigate nano-TiO 2 toxicity and the modifying factors thereof based on the paradigm of oxidative stress and to explore the utility of extracellular H 2 O 2 as a potential biomarker of the observed cellular responses. This is the first study to provide continuous quantitative data on abiotic and biotic nano-TiO 2 -driven H 2 O 2 generation to systematically investigate the link between extracellular and cellular pro-oxidant responses. Acute exposures of 1 h were performed in two different exposure media (MOPS and lake water), with nominal particle concentrations from 10 mg L −1 to 200 mg L −1 , with and without UV pre-illumination. Abiotic and biotic extracellular H 2 O 2 were continuously measured with the biosensor and complemented with endpoints for abiotic ROS (H 2 DCF-DA), oxidative stress (CellROX® Green) and damage (propidium iodide) measured by flow cytometry at the beginning and end of exposure. Results showed that nano-TiO 2 suspensions generated ROS under UV light (abiotic origin) and promoted ROS accumulation in C. reinhardtii (biotic origin). However, extracellular and intracellular pro-oxidant processes differed. Hence, extracellular H 2 O 2 cannot per se serve as a predictor of cellular oxidative stress or damage. The main predictors best describing the cellular responses included “exposure medium”, “exposure time”, “UV treatment” as well as “exposure concentration”.