A direct and simple method for rapidly counting viable chains of Leuconostoc in batch cultures

Plating determines the concentration in viable cells of bacteria but remains time-consuming and inaccurate. A direct and simple method combining two techniques, haemacytometry and epifluorescence, gives both the viability and the concentration in viable cells. The method was evaluated using Leuconos...

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Veröffentlicht in:	Biotechnology techniques 1995-06, Vol.9 (6), p.451-456
Hauptverfasser:	PLIHON, F, TAILLANDIER, P, STREHAIANO, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Biotechnology Chemical and Process Engineering Chemical engineering Chemical Sciences Engineering Sciences Fundamental and applied biological sciences. Psychology Leuconostoc mesenteroides Methods. Procedures. Technologies Microbiology Others Various methods and equipments
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Beschreibung
Zusammenfassung:	Plating determines the concentration in viable cells of bacteria but remains time-consuming and inaccurate. A direct and simple method combining two techniques, haemacytometry and epifluorescence, gives both the viability and the concentration in viable cells. The method was evaluated using Leuconostoc mesenteroides.
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF00160835