Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts

Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts

A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parvum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocyst/cell ratio and the incubation time. The infection rates did not...

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Veröffentlicht in:Journal of microbiological methods 2001-07, Vol.46 (1), p.81-84
Hauptverfasser: Lawton, Philippe, Mancassola, Roselyne, Naciri, Muriel, Pétavy, Anne-Françoise
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bacterial diseases
Bacteriology
Biological and medical sciences
Cell Line
Cells, Cultured
Cryptosporidium parvum
Cryptosporidium parvum - growth & development
Cryptosporidium parvum - pathogenicity
Culture
excystation
Experimental bacterial diseases and models
Fundamental and applied biological sciences. Psychology
In vitro
In Vitro Techniques
Infectious diseases
Life Sciences
MDBK
MDBK cells
Medical sciences
Microbiology
Microbiology and Parasitology
Ovum - growth & development
Parasite Egg Count
Parasitology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Percoll
Povidone
Silicon Dioxide
THP-1
THP-1 cells
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Zusammenfassung:A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parvum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocyst/cell ratio and the incubation time. The infection rates did not increase with the oocyst/cell ratio and both cell types were equally parasitized.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(01)00265-2