Photothermal effect for localized desorption of primary lymphocytes arrayed on an antibody/DNA-based biochip

This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a biochip surface. Unlabelled lymphocytes were first specifically captured onto a DNA array by antibody-DNA conjugates. The immobilized cells were subsequently released under s...

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Veröffentlicht in:	Lab on a chip 2014-06, Vol.14 (12), p.1987-1990
Hauptverfasser:	Leroy, Loïc, Bombera, Radoslaw, Engel, Elodie, Calemczuk, Roberto, Laplatine, Loïc, Baganizi, Dieu-donné R, Marche, Patrice N, Roupioz, Yoann, Livache, Thierry
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - chemistry B-Lymphocytes - chemistry B-Lymphocytes - metabolism Biotechnology Cells, Immobilized - cytology Cells, Immobilized - metabolism Chemical Sciences DNA - chemistry Life Sciences Mice Organic chemistry Surface Plasmon Resonance - instrumentation Surface Plasmon Resonance - methods T-Lymphocytes - cytology T-Lymphocytes - metabolism Tissue Array Analysis - instrumentation Tissue Array Analysis - methods
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Beschreibung
Zusammenfassung:	This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a biochip surface. Unlabelled lymphocytes were first specifically captured onto a DNA array by antibody-DNA conjugates. The immobilized cells were subsequently released under spatiotemporal control within local heating generated by intense Surface Plasmon Resonance (SPR) produced by laser illumination.
ISSN:	1473-0197 1473-0189
DOI:	10.1039/c4lc00336e