Environmental Toxicity and Antimicrobial Efficiency of Titanium Dioxide Nanoparticles in Suspension

The aim of this work was to evaluate the photokilling efficiency of synthesized titanium dioxide nanoparticles in suspension. Two strains of Escherichia coli, Lactobacillus casei rhamnosus and Staphylococcus aureus were used as probes to test the photokilling activities of the nanoparticles. The toxicity effects of TiO 2 nanoparticles on the environment were determined by a standard test using gram-negative bioluminescent bacteria Vibrio fischeri. The antimicrobial activity of these nanoparticles (NPs) was then investigated versus NPs concentration, UV irradiation time and mi-cro-organism strains. We evaluated the LC50 values of the nanoparticles suspension by counting the Colony-Forming Units. Results highlighted the differences in bacteria sensitivity facing photo-killing treatment induced by the irradiation of anatase TiO 2 nanoparticles suspension. At the concentration of 1 g·L −1 TiO 2 , tested bacteria were killed after 30 minutes of photo-treatment. Using different TiO 2 concentrations, the Staphylococcus aureus gram-positive/catalase-positive bacteria were more resistant than gram-negative/catalase-positive ones or gram-positive/catalase-negative bacteria. An effect of UV irradiation was evaluated by the quantification of hydrogen peroxide generated by the photolysis of water molecules in presence of the nanoparticles with or without the most resistant bacterium (S. aureus). After 30 minutes with UV irradiation in these two conditions , the concentration of hydrogen peroxide was 35 µM in presence of 1.2 g·L −1 TiO 2 suspension. This result suggested that the resistance mechanism of S. aureus was not due to an extracelullar H 2 O 2 enzymatic degradation.