Interlaboratory quality control of total HIV‐1 DNA load measurement for multicenter reservoir studies

Background Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten l...

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Veröffentlicht in:	Journal of medical virology 2017-11, Vol.89 (11), p.2047-2050
Hauptverfasser:	Gantner, Pierre, Mélard, Adeline, Damond, Florence, Delaugerre, Constance, Dina, Julia, Gueudin, Marie, Maillard, Anne, Sauné, Karine, Rodallec, Audrey, Tuaillon, Edouard, Plantier, Jean‐Christophe, Rouzioux, Christine, Avettand‐Fenoel, Véronique
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Sprache:	eng
Schlagworte:	Clinical Laboratory Techniques - methods Clinical Laboratory Techniques - standards Deoxyribonucleic acid Disease Reservoirs - virology DNA DNA, Viral - analysis Feasibility studies HIV HIV-1 - genetics HIV-1 - isolation & purification HIV-1 - physiology HIV‐1 DNA Human immunodeficiency virus Humans interlaboratory quality control Intersectoral Collaboration Laboratories Life Sciences Peripheral blood mononuclear cells Quality Control quantification Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reproducibility Reproducibility of Results RNA, Viral - blood Viral Load - standards Virology
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container_title	Journal of medical virology
container_volume	89
creator	Gantner, Pierre Mélard, Adeline Damond, Florence Delaugerre, Constance Dina, Julia Gueudin, Marie Maillard, Anne Sauné, Karine Rodallec, Audrey Tuaillon, Edouard Plantier, Jean‐Christophe Rouzioux, Christine Avettand‐Fenoel, Véronique
description	Background Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten laboratories of the ANRS‐AC11 working group participated by quantifying HIV‐DNA with a real‐time qPCR assay (Biocentric) in four samples (QCMD). Results Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1
doi_str_mv	10.1002/jmv.24874
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Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten laboratories of the ANRS‐AC11 working group participated by quantifying HIV‐DNA with a real‐time qPCR assay (Biocentric) in four samples (QCMD). Results Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1<QC2<QC3). Conclusions Results of this QC validate the feasibility of multicenter studies using this standardized assay.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.24874</identifier><identifier>PMID: 28617961</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Clinical Laboratory Techniques - methods ; Clinical Laboratory Techniques - standards ; Deoxyribonucleic acid ; Disease Reservoirs - virology ; DNA ; DNA, Viral - analysis ; Feasibility studies ; HIV ; HIV-1 - genetics ; HIV-1 - isolation & purification ; HIV-1 - physiology ; HIV‐1 DNA ; Human immunodeficiency virus ; Humans ; interlaboratory quality control ; Intersectoral Collaboration ; Laboratories ; Life Sciences ; Peripheral blood mononuclear cells ; Quality Control ; quantification ; Real-Time Polymerase Chain Reaction - methods ; Real-Time Polymerase Chain Reaction - standards ; Reproducibility ; Reproducibility of Results ; RNA, Viral - blood ; Viral Load - standards ; Virology</subject><ispartof>Journal of medical virology, 2017-11, Vol.89 (11), p.2047-2050</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4224-83b30ff0fe6475f8f6227a44370abe5ea9afd68104927aeab1d67c724126c41e3</citedby><cites>FETCH-LOGICAL-c4224-83b30ff0fe6475f8f6227a44370abe5ea9afd68104927aeab1d67c724126c41e3</cites><orcidid>0000-0002-7022-2990 ; 0000-0001-6417-4083</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.24874$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.24874$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28617961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01760708$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gantner, Pierre</creatorcontrib><creatorcontrib>Mélard, Adeline</creatorcontrib><creatorcontrib>Damond, Florence</creatorcontrib><creatorcontrib>Delaugerre, Constance</creatorcontrib><creatorcontrib>Dina, Julia</creatorcontrib><creatorcontrib>Gueudin, Marie</creatorcontrib><creatorcontrib>Maillard, Anne</creatorcontrib><creatorcontrib>Sauné, Karine</creatorcontrib><creatorcontrib>Rodallec, Audrey</creatorcontrib><creatorcontrib>Tuaillon, Edouard</creatorcontrib><creatorcontrib>Plantier, Jean‐Christophe</creatorcontrib><creatorcontrib>Rouzioux, Christine</creatorcontrib><creatorcontrib>Avettand‐Fenoel, Véronique</creatorcontrib><creatorcontrib>ANRS-AC11 Quantification Working Group</creatorcontrib><creatorcontrib>on behalf of the ANRS-AC11 Quantification Working Group</creatorcontrib><title>Interlaboratory quality control of total HIV‐1 DNA load measurement for multicenter reservoir studies</title><title>Journal of medical virology</title><addtitle>J Med Virol</addtitle><description>Background Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten laboratories of the ANRS‐AC11 working group participated by quantifying HIV‐DNA with a real‐time qPCR assay (Biocentric) in four samples (QCMD). Results Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1<QC2<QC3). 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Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten laboratories of the ANRS‐AC11 working group participated by quantifying HIV‐DNA with a real‐time qPCR assay (Biocentric) in four samples (QCMD). Results Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1<QC2<QC3). Conclusions Results of this QC validate the feasibility of multicenter studies using this standardized assay.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28617961</pmid><doi>10.1002/jmv.24874</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-7022-2990</orcidid><orcidid>https://orcid.org/0000-0001-6417-4083</orcidid><oa>free_for_read</oa></addata></record>
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subjects	Clinical Laboratory Techniques - methods Clinical Laboratory Techniques - standards Deoxyribonucleic acid Disease Reservoirs - virology DNA DNA, Viral - analysis Feasibility studies HIV HIV-1 - genetics HIV-1 - isolation & purification HIV-1 - physiology HIV‐1 DNA Human immunodeficiency virus Humans interlaboratory quality control Intersectoral Collaboration Laboratories Life Sciences Peripheral blood mononuclear cells Quality Control quantification Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reproducibility Reproducibility of Results RNA, Viral - blood Viral Load - standards Virology
title	Interlaboratory quality control of total HIV‐1 DNA load measurement for multicenter reservoir studies
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