Interlaboratory quality control of total HIV‐1 DNA load measurement for multicenter reservoir studies

Background Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten l...

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Veröffentlicht in:	Journal of medical virology 2017-11, Vol.89 (11), p.2047-2050
Hauptverfasser:	Gantner, Pierre, Mélard, Adeline, Damond, Florence, Delaugerre, Constance, Dina, Julia, Gueudin, Marie, Maillard, Anne, Sauné, Karine, Rodallec, Audrey, Tuaillon, Edouard, Plantier, Jean‐Christophe, Rouzioux, Christine, Avettand‐Fenoel, Véronique
Format:	Artikel
Sprache:	eng
Schlagworte:	Clinical Laboratory Techniques - methods Clinical Laboratory Techniques - standards Deoxyribonucleic acid Disease Reservoirs - virology DNA DNA, Viral - analysis Feasibility studies HIV HIV-1 - genetics HIV-1 - isolation & purification HIV-1 - physiology HIV‐1 DNA Human immunodeficiency virus Humans interlaboratory quality control Intersectoral Collaboration Laboratories Life Sciences Peripheral blood mononuclear cells Quality Control quantification Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reproducibility Reproducibility of Results RNA, Viral - blood Viral Load - standards Virology
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Zusammenfassung:	Background Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter‐laboratory reproducibility of total HIV‐1‐DNA quantification. Methods Ten laboratories of the ANRS‐AC11 working group participated by quantifying HIV‐DNA with a real‐time qPCR assay (Biocentric) in four samples (QCMD). Results Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1
ISSN:	0146-6615 1096-9071
DOI:	10.1002/jmv.24874