Supercontinuum-based Fourier transform infrared spectromicroscopy

Fourier-transform infrared (FTIR) spectromicroscopy combines the spatial resolution of optical microscopy with the spectral selectivity of vibrational spectroscopy. Synchrotron sources can provide diffraction-limited beams in the infrared, and therefore synchrotron-based FTIR spectromicroscopy is nowadays an indispensable tool for biology and materials science studies where high spatial resolution is required. However, the increasing need for accurate and highly spatially resolved characterization is calling for alternative laboratory-based sources to complement synchrotron radiation. To date, the low brightness of thermal emitters or high temporal coherence and narrow bandwidth or tunability of laser sources have hindered the progress of bench-top FTIR spectromicroscopy. Here, we demonstrate that fiber-based supercontinuum sources in the mid-infrared enable fast spectral mapping of localized material properties with close to diffraction-limited resolution (3 μm×3 μm) and pave the way to table-top, on-demand, fast, and highly spatially resolved studies. We illustrate these capabilities by imaging thin sections of human liver samples and compare the results and performance with those obtained using a synchrotron source.