Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm

It was hypothesized that dimethyleacetamide (DMA) can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl semen. Four concentrations of DMA (4%, 6%, 8% and 10%) in extender were compared with previously optimized cryopreservation protocol based on 20% glycerol (control...

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Veröffentlicht in:	Theriogenology 2017-11, Vol.103, p.83-89
Hauptverfasser:	Rakha, B.A., Ansari, M.S., Akhter, S., Zafar, Z., Naseer, A., Hussain, I., Santiago-Moreno, J., Blesbois, E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetamides - administration & dosage Acetamides - pharmacology Acrosome Animals Chickens Cryo-damage Cryopreservation - veterinary Cryoprotective Agents - administration & dosage Cryoprotective Agents - pharmacology Dose-Response Relationship, Drug Fertility Freezing Indian red jungle fowl Life Sciences Male Motility Other Semen Analysis - veterinary Semen Preservation - methods Sperm Motility - drug effects Sperm viability Spermatozoa - drug effects
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Zusammenfassung:	It was hypothesized that dimethyleacetamide (DMA) can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl semen. Four concentrations of DMA (4%, 6%, 8% and 10%) in extender were compared with previously optimized cryopreservation protocol based on 20% glycerol (control) for Indian red jungle fowl. Sperm motility, plasma membrane integrity, viability, and acrosome integrity were assessed at the stage of post-dilution, cooling, equilibration, and freeze-thawing. The whole experiment was repeated/replicated for five times independently. Sperm motility, plasma membrane integrity, viability and acrosome integrity were recorded highest (P
ISSN:	0093-691X 1879-3231
DOI:	10.1016/j.theriogenology.2017.07.018