Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-01, Vol.49 (51), p.5781-5783
Hauptverfasser: Abdellaoui, Sofiène, Bekhouche, Mourad, Noiriel, Alexandre, Henkens, Robert, Bonaventura, Celia, Blum, Loïc J, Doumèche, Bastien
Format: Artikel
Sprache:eng
Schlagworte:
Biocatalysis
Biochemistry
Biochemistry, Molecular Biology
Candida - enzymology
Carbon - chemistry
Chemical Sciences
Directed Molecular Evolution
Electrochemical Techniques
Electrodes
Formate Dehydrogenases - genetics
Formate Dehydrogenases - metabolism
Life Sciences
Mutation
NAD - chemistry
Organic chemistry
Phenazines - chemistry
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Beschreibung
Zusammenfassung:The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc42065e