Prefolded Synthetic G-Quartets Display Enhanced Bioinspired Properties

A water‐soluble template‐assembled synthetic G‐quartet (TASQ) based on the use of a macrocyclodecapeptide scaffold was designed to display stable intramolecular folds alone in solution. The preformation of the guanine quartet, demonstrated by NMR and CD investigations, results in enhanced peroxidase‐type biocatalytic activities and improved quadruplex‐interacting properties. Comparison of its DNAzyme‐boosting properties with the ones of previously published TASQ revealed that, nowadays, it is the best DNAzyme‐boosting agent. Significance of preformation: A water‐soluble template‐assembled synthetic G‐quartet (TASQ) has been prepared. It allows the formation of a stable and isolated G‐quartet in solution resulting in enhanced peroxidase‐type biocatalytic activities and improved quadruplex‐interacting properties.