Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.

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Bibliographische Detailangaben
Veröffentlicht in:	Molecular and cellular probes 2014-02, Vol.28 (1), p.10-12
Hauptverfasser:	Costaglioli, Patricia, Barthe, Christophe, Fayon, Michael, Christoflour, Naik, Bui, Stéphanie, Derlich, Laurence, Domblides, Philippe, Crouzet, Marc, Vilain, Sébastien, Garbay, Bertrand
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemical Sciences Cystic fibrosis Cystic Fibrosis - microbiology Gene Expression Profiling - methods Genes, Bacterial Humans Polymers Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Real-Time Polymerase Chain Reaction Reference genes RT-qPCR Sputum Sputum - microbiology
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Beschreibung
Zusammenfassung:	The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.
ISSN:	0890-8508 1096-1194
DOI:	10.1016/j.mcp.2013.09.003