Nanoparticles Self-Assembly Driven by High Affinity Repeat Protein Pairing
Proteins are the most specific yet versatile biological self-assembling agents with a rich chemistry. Nevertheless, the design of new proteins with recognition capacities is still in its infancy and has seldom been exploited for the self-assembly of functional inorganic nanoparticles. Here, we repor...
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Veröffentlicht in: | ACS nano 2016-03, Vol.10 (3), p.3176-3185 |
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creator | Gurunatha, Kargal L Fournier, Agathe C Urvoas, Agathe Valerio-Lepiniec, Marie Marchi, Valérie Minard, Philippe Dujardin, Erik |
description | Proteins are the most specific yet versatile biological self-assembling agents with a rich chemistry. Nevertheless, the design of new proteins with recognition capacities is still in its infancy and has seldom been exploited for the self-assembly of functional inorganic nanoparticles. Here, we report on the protein-directed assembly of gold nanoparticles using purpose-designed artificial repeat proteins having a rigid but modular 3D architecture. αRep protein pairs are selected for their high mutual affinity from a library of 109 variants. Their conjugation onto gold nanoparticles drives the massive colloidal assembly of free-standing, one-particle thick films. When the average number of proteins per nanoparticle is lowered, the extent of self-assembly is limited to oligomeric particle clusters. Finally, we demonstrate that the aggregates are reversibly disassembled by an excess of one free protein. Our approach could be optimized for applications in biosensing, cell targeting, or functional nanomaterials engineering. |
doi_str_mv | 10.1021/acsnano.5b04531 |
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Our approach could be optimized for applications in biosensing, cell targeting, or functional nanomaterials engineering.</description><subject>Chemical Sciences</subject><subject>Gold - chemistry</subject><subject>Material chemistry</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Models, Molecular</subject><subject>Nanotechnology - methods</subject><subject>Proteins - chemistry</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kNtLwzAUh4MoTqfPvkleRbrl0ibpY5mXKUOHF_CtJFmyZXRtSbpB_3s7Ovfm0zkcvu9wzg-AG4xGGBE8ljqUsqxGiUJxQvEJuMApZRES7Of02Cd4AC5DWCOUcMHZORgQJhglQlyA17dOr6VvnC5MgJ-msFEWgtmoooUP3u1MCVULp265gpm1rnRNCz9MbWQD575qjCvhXDrvyuUVOLOyCOb6UIfg--nxazKNZu_PL5NsFsmYx02ENYo1E1hpQqW1eqETmhCbME0l7Q5HSpI0xUISypVAlscpx1YybhmTiqR0CO76vStZ5LV3G-nbvJIun2azfD9DmPBY0HiHO3bcs9pXIXhjjwJG-T7B_JBgfkiwM257o96qjVkc-b_IOuC-BzozX1dbX3bP_rvuF-34e0I</recordid><startdate>20160322</startdate><enddate>20160322</enddate><creator>Gurunatha, Kargal L</creator><creator>Fournier, Agathe C</creator><creator>Urvoas, Agathe</creator><creator>Valerio-Lepiniec, Marie</creator><creator>Marchi, Valérie</creator><creator>Minard, Philippe</creator><creator>Dujardin, Erik</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-7242-9250</orcidid></search><sort><creationdate>20160322</creationdate><title>Nanoparticles Self-Assembly Driven by High Affinity Repeat Protein Pairing</title><author>Gurunatha, Kargal L ; Fournier, Agathe C ; Urvoas, Agathe ; Valerio-Lepiniec, Marie ; Marchi, Valérie ; Minard, Philippe ; Dujardin, Erik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a474t-1c04c681bc23affcdc5352f56c3a30860ba29918a237b80f74971fa67f66ab293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Chemical Sciences</topic><topic>Gold - chemistry</topic><topic>Material chemistry</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Models, Molecular</topic><topic>Nanotechnology - methods</topic><topic>Proteins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gurunatha, Kargal L</creatorcontrib><creatorcontrib>Fournier, Agathe C</creatorcontrib><creatorcontrib>Urvoas, Agathe</creatorcontrib><creatorcontrib>Valerio-Lepiniec, Marie</creatorcontrib><creatorcontrib>Marchi, Valérie</creatorcontrib><creatorcontrib>Minard, Philippe</creatorcontrib><creatorcontrib>Dujardin, Erik</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gurunatha, Kargal L</au><au>Fournier, Agathe C</au><au>Urvoas, Agathe</au><au>Valerio-Lepiniec, Marie</au><au>Marchi, Valérie</au><au>Minard, Philippe</au><au>Dujardin, Erik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanoparticles Self-Assembly Driven by High Affinity Repeat Protein Pairing</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2016-03-22</date><risdate>2016</risdate><volume>10</volume><issue>3</issue><spage>3176</spage><epage>3185</epage><pages>3176-3185</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>Proteins are the most specific yet versatile biological self-assembling agents with a rich chemistry. 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subjects | Chemical Sciences Gold - chemistry Material chemistry Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Models, Molecular Nanotechnology - methods Proteins - chemistry |
title | Nanoparticles Self-Assembly Driven by High Affinity Repeat Protein Pairing |
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