Staphylococcus aureus transcriptomic response to inhibition by H2O2-producing Lactococcus garvieae

Growth of the foodborne pathogen Staphylococcus aureus can be inhibited in milk and in cheese by the hydrogen peroxide-producing Lactococcus garvieae N201 dairy strain. Transcriptomic responses of two S. aureus strains, the S. aureus SA15 dairy strain and the MW2 human pathogenic strain, to this gro...

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Veröffentlicht in:Food microbiology 2015-10, Vol.51, p.163-170
Hauptverfasser: Delpech, Pierre, Bornes, Stéphanie, Alaterre, Elina, Bonnet, Muriel, Gagne, Geneviève, Montel, Marie-Christine, Delbès, Céline
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Sprache:eng
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Zusammenfassung:Growth of the foodborne pathogen Staphylococcus aureus can be inhibited in milk and in cheese by the hydrogen peroxide-producing Lactococcus garvieae N201 dairy strain. Transcriptomic responses of two S. aureus strains, the S. aureus SA15 dairy strain and the MW2 human pathogenic strain, to this growth inhibition were investigated in Brain-Heart Infusion broth under a high or a low aeration level. We demonstrated that S. aureus MW2 had a higher resistance to L. garvieae inhibition under the high aeration level: this correlated to a higher survival under hydrogen peroxide exposure. Conversely, the two strains were similarly inhibited under the low aeration level. Expression of S. aureus genes involved in response to H2O2 or other stresses as well as in cell division was generally repressed by L. garvieae. However, differential expressions between the two S. aureus strains were observed, especially under the high aeration level. Additionally, expression of virulence-related genes (enterotoxins, regulatory genes) was modulated by L. garvieae depending on the aeration level and on the S. aureus strain. This study led to new insights into potential molecular mechanisms of S. aureus inhibition by Lactic Acid Bacteria via H2O2 production. •Staphylococcus aureus inhibition was higher when Lactococcus garvieae produces hydrogen peroxide (H2O2).•Pathogenic MW2 strain was inhibited less than dairy SA15 strain under H2O2 exposure.•S. aureus inhibition was associated with stress and H2O2 response genes repression.•S. aureus inhibition was associated with cell division genes repression.•S. aureus inhibition was associated with modulation of virulence and regulatory genes.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2015.05.014