The FTIR spectroscopy investigation of the cellular components of cassava after sensitization with plant growth promoting rhizobacteria, [i]Bacillus subtili[/i]s CaSUT007

To evaluate the response of cassava stakes to plant growth promoting rhizobacteria, Bacillus subtilis CaSUT007, the changes in cellular compositions and phytohormone were investigated using the fourier transform infrared (FTIR) and high-performance liquid chromatography (HPLC) approach. The objective of this study was to test the hypothesis that CaSUT007 stimulates production of plant cellular components and phytohormone involved in metabolism and growth development mechanisms. Cassava stake treated with CaSUT007 or with sterile distilled water were germinated in sterile soil, after incubation for 28 days, CaSUT007 treated cassava stakes had more lateral root, longer roots, shoot length and greater biomass than the control which enhanced more than 1.3 fold of the cassava's phytohormone as indole-3-acetic acid content of non-treated control. We also focused on plant cellular composition and cassava stake tissues from the two treatments were harvested for FTIR analysis. FTIR analyses revealed that higher accumulated of lipid in response to the strain CaSUT007. The cassava stake treated with the beneficial bacteria B. subtilis strain CaSUT007 showed the higher content of the lipid content as (shown in the spectral regions of CH stretching and CH bending mode associated with cell membrane structure lipids) when compared with those of the cassava stake treated with distilled water. Our results initially demonstrated that CaSUT007 can enhance plant growth under greenhouse conditions by direct stimulation of plant lipid and phytohormone as indole-3-acetic acid production.