Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase

Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase

Erwinia chrysanthemi produced several pectate lyases (EC 4.2.2.2) and endocellulases (EC 3.2.1.4) which were largely secreted into the culture medium. Mutants deficient in the secretion mechanism for these enzymes were obtained by chemical and insertion mutagenesis. Further study of one such mutant...

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Veröffentlicht in:Journal of bacteriology 2014, Vol.160 (3), p.1199-1203
Hauptverfasser: Andro, Thierry, Chambost, Jean-Pierre, Kotoujansky, Alain, Cattaneo, Jeanne, Bertheau, Yves, Barras, Frédéric, van Gijsegem, Frédérique, Coleno, Alain
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Sprache:eng
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Zusammenfassung:Erwinia chrysanthemi produced several pectate lyases (EC 4.2.2.2) and endocellulases (EC 3.2.1.4) which were largely secreted into the culture medium. Mutants deficient in the secretion mechanism for these enzymes were obtained by chemical and insertion mutagenesis. Further study of one such mutant revealed that both enzyme activities were retained simultaneously within the periplasmic space.
ISSN:0021-9193
1098-5530