Queen production and instrumental insemination of Apis florea queens

Virgin queens of A . florea were produced in 10 queenless colonies yielding 106 queens with an average of 10.6 ± 2.99 queen cells per colony and a success rate of 65.23 ± 0.14% virgin queens. Spermatozoa were collected directly from the seminal vesicles. Thirty queens were inseminated, each with a p...

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Veröffentlicht in:Apidologie 2011-05, Vol.42 (3), p.307-311
Hauptverfasser: Phiancharoen, Mananya, Wongsiri, Siriwat, Hepburn, H. Randall
Format: Artikel
Sprache:eng
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Zusammenfassung:Virgin queens of A . florea were produced in 10 queenless colonies yielding 106 queens with an average of 10.6 ± 2.99 queen cells per colony and a success rate of 65.23 ± 0.14% virgin queens. Spermatozoa were collected directly from the seminal vesicles. Thirty queens were inseminated, each with a pool of about 3.12 × 10 6 spermatozoa derived from 8 drones. Six queens began to lay eggs 5 to 14 days after instrumental insemination. The mean number of spermatozoa reaching the spermatheca of inseminated queens was 0.74 × 10 6  ± 0.45 (=24% of the drone’s spermatozoa) and the percentage of worker offspring was 100% in 5 queens and 83% in one queen. This method opens the possibility for new studies in genetics and selective breeding.
ISSN:0044-8435
1297-9678
DOI:10.1007/s13592-011-0068-y