Dissociation Kinetics of a Binary Complex in Solution by Protein Displacement

Kinetics determined by NMR: Whereas most measurements of kinetic parameters of a ligand–protein complex use displacement of the ligand, protein displacement can give accurate off‐rates in solution (see picture). Analytical and simulation results are given for the apparent off‐rate obtained by protei...

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Veröffentlicht in:	Angewandte Chemie International Edition 2013-11, Vol.52 (48), p.12587-12591
Hauptverfasser:	Launay, Helene, Parent, Benjamin, Page, Adeline, Hanoulle, Xavier, Lippens, Guy
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biochemistry, Molecular Biology Cyclophilin A - chemistry Cyclophilin A - metabolism Cyclosporine - chemistry Cyclosporine - metabolism Displacement Immunosuppressive Agents - chemistry Immunosuppressive Agents - metabolism Kinetics Life Sciences ligand-protein complexes Ligands Mathematical analysis Models, Molecular NMR spectroscopy Nuclear magnetic resonance Nuclear Magnetic Resonance, Biomolecular off-rate constants Pictures Proteins Simulation
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Beschreibung
Zusammenfassung:	Kinetics determined by NMR: Whereas most measurements of kinetic parameters of a ligand–protein complex use displacement of the ligand, protein displacement can give accurate off‐rates in solution (see picture). Analytical and simulation results are given for the apparent off‐rate obtained by protein displacement.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201306253