Chalkbrood development in honeybee brood under controlled conditions
Third instar larvae from a honeybee colony were fed with high doses of spores of Ascosphaera apis, the causative agent of chalkbrood disease. Optimal survival of spores was detected during a short period after sealing the cell and before larval spinning by culture of the gut contents removed from 4...
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Veröffentlicht in: | Apidologie 1994, Vol.25 (6), p.540-546 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Third instar larvae from a honeybee colony were fed with high doses of spores of Ascosphaera apis, the causative agent of chalkbrood disease. Optimal survival of spores was detected during a short period after sealing the cell and before larval spinning by culture of the gut contents removed from 4 stages of brood development. The inocula (5 x 10 super(5) spores/larva) did not induce the disease and were not present in the digestive tract before pupation. In a second experiment, third instar larvae, fed with the same amounts of spores as before, received a cooling stress (22 plus or minus 2 degree C, for 24 h). When chilling was applied 24 h before or after operculation, mummification occurred in the majority of larvae (59.6 and 65.5%, respectively). Chilling of older brood (spinning larvae or pupa) produced a much lower incidence of chalkbrood. This confirms the need for predisposing conditions over a short period of brood development for the development of this disease. |
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ISSN: | 0044-8435 1297-9678 |
DOI: | 10.1051/apido:19940604 |