Chalkbrood development in honeybee brood under controlled conditions

Third instar larvae from a honeybee colony were fed with high doses of spores of Ascosphaera apis, the causative agent of chalkbrood disease. Optimal survival of spores was detected during a short period after sealing the cell and before larval spinning by culture of the gut contents removed from 4...

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Veröffentlicht in:Apidologie 1994, Vol.25 (6), p.540-546
Hauptverfasser: Puerta, F. (Universidad de Cordoba (Espagne). Facultad de Veterinaria, Catedra de Biologia Aplicada, Departamento de Biologia Animal, Seccion de Apicultura), Flores, J.M, Buston, M, Padilla, F, Campano, F
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Sprache:eng
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Zusammenfassung:Third instar larvae from a honeybee colony were fed with high doses of spores of Ascosphaera apis, the causative agent of chalkbrood disease. Optimal survival of spores was detected during a short period after sealing the cell and before larval spinning by culture of the gut contents removed from 4 stages of brood development. The inocula (5 x 10 super(5) spores/larva) did not induce the disease and were not present in the digestive tract before pupation. In a second experiment, third instar larvae, fed with the same amounts of spores as before, received a cooling stress (22 plus or minus 2 degree C, for 24 h). When chilling was applied 24 h before or after operculation, mummification occurred in the majority of larvae (59.6 and 65.5%, respectively). Chilling of older brood (spinning larvae or pupa) produced a much lower incidence of chalkbrood. This confirms the need for predisposing conditions over a short period of brood development for the development of this disease.
ISSN:0044-8435
1297-9678
DOI:10.1051/apido:19940604