Enhanced Colorimetric Detection on Porous Microarrays Using in Situ Substrate Production

A new technique is reported for the enhanced colorimetric detection of multiplexed hybridization onto porous membrane-based microarrays. This approach combines the use of horseradish peroxidase (HRP) as a label together with a chromogen substrate and a local production of the hydrogen peroxide requi...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-05, Vol.83 (9), p.3610-3615
Hauptverfasser:	Le Goff, Gaelle C, Blum, Loïc J, Marquette, Christophe A
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Aspergillus niger - enzymology Base Sequence Benzidines - metabolism Blood Group Antigens - genetics Cattle Chemical compounds Chemical Sciences Chemistry Colorimetry - methods Exact sciences and technology General, instrumentation Genotype Glucose Glucose Oxidase - metabolism Horseradish Peroxidase - metabolism Humans Hydrogen peroxide Hydrogen Peroxide - metabolism Indoles - metabolism Membranes Membranes, Artificial Microarray Analysis - methods Nitroblue Tetrazolium - metabolism Oxidation Polymerase Chain Reaction Polymorphism Polymorphism, Single Nucleotide - genetics Porosity Spectrometric and optical methods
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