A FRET-based probe with a chemically deactivatable quencher

A FRET-based probe with a chemically deactivatable quencher

A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted pr...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2012-01, Vol.48 (26), p.3224-3226
Hauptverfasser: Leriche, Geoffray, Budin, Ghyslain, Darwich, Zeinab, Weltin, Denis, Mély, Yves, Klymchenko, Andrey S, Wagner, Alain
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry, Molecular Biology
Caspase 3 - chemistry
Caspase 3 - metabolism
Cellular Biology
Dithionite - chemistry
Fluorescence
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Life Sciences
Molecular Structure
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
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Zusammenfassung:A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments.
ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc17542h