O-GlcNAcylation is a novel regulator of lung and colon cancer malignancy

O-GlcNAc is a monosaccharide attached to serine or threonine hydroxyl moieties on numerous nuclear and cytoplasmic proteins; O-GlcNAcylation is dynamically regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Although recent studies have shown that O-GlcNAcylation plays essential roles in...

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Veröffentlicht in:Biochimica et biophysica acta 2011-04, Vol.1812 (4), p.514-519
Hauptverfasser: Mi, Wenyi, Gu, Yuchao, Han, Cuifang, Liu, Haiyan, Fan, Qiong, Zhang, Xinling, Cong, Qi, Yu, Wengong
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Sprache:eng
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Zusammenfassung:O-GlcNAc is a monosaccharide attached to serine or threonine hydroxyl moieties on numerous nuclear and cytoplasmic proteins; O-GlcNAcylation is dynamically regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Although recent studies have shown that O-GlcNAcylation plays essential roles in breast cancer progression, it is also necessary to know whether O-GlcNAcylation is involved in other types of human cancer. In this study, O-GlcNAcylation levels and the expressions of OGT and OGA in human lung and colon cancer tissues were examined by immunohistochemistry analysis. We found that O-GlcNAcylation as well as OGT expression was significantly elevated in the cancer tissues compared with that in the corresponding adjacent tissues. Additionally, the roles of O-GlcNAcylation in the malignancy of lung and colon cancer were investigated in vitro. The results showed that O-GlcNAcylation markedly enhanced the anchorage-independent growth of lung and colon cancer cells; O-GlcNAcylation could also enhance lung and colon cancer invasion in a context-dependent manner. All together, this study suggests that O-GlcNAcylation might play important roles in lung and colon cancer formation and progression, and may be a valuable target for diagnosis and therapy of cancer. ►The levels of O-GlcNAc and OGT were elevated in the lung and colon cancer tissues. ►OGT increase may be a main cause of O-GlcNAc elevation in tumor tissues. ►O-GlcNAc plays important roles in lung and colon cancer malignancy.
ISSN:0925-4439
0006-3002
1879-260X
DOI:10.1016/j.bbadis.2011.01.009