Biochip for astrobiological applications: Investigation of low energy protons effects on antibody performances

Antibody-based micro-arrays instruments are very promising tools for the search for biomarkers in planetary exploration missions. Since such instruments have never been used in this context, it is important to test their resistance to space constraints. In particular, cosmic particles might be deleterious. In the present study, we have investigated the effect of low energy protons (2MeV) on antibody performances with fluences levels much greater than expected for a typical mission to Mars. We show that these particles do not alter significantly the antibody recognition capability for both free (in solution) and grafted (covalently bound to the support) freeze–dried antibodies. Details of the freeze–dried drying process used to optimize antibody performances during our experiments are also presented. ► Resistance under low energy protons (2MeV) of antibody-based micro-arrays instruments. ► Antibodies were irradiated under three freeze–dried conditions. ► No proton irradiation effect were observed. ► Need to encompass all the constraints encountered during a planetary mission. ► Further experiments will be performed using International space station facilities.