1-Ethyl-3-Methylimidazolium Ethylsulfate/Copper Catalyst for the Enhancement of Glucose Chemiluminescent Detection: Effects on Light Emission and Enzyme Activity

The effect of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO4]) on the copper-catalyzed luminol chemiluminescence (CL) is reported. A drastic light emission enhancement is observed, related to a strong interaction between Cu2+ and the imidazolium ring. In these conditions, th...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-03, Vol.82 (6), p.2401-2404
Hauptverfasser:	Santafé, Aurélie A.-M, Doumèche, Bastien, Blum, Loïc J, Girard-Egrot, Agnès P, Marquette, Christophe A
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidity Analytical chemistry Aspergillus niger - enzymology Biochemistry, Molecular Biology Catalysis Catalysts Chemical and thermal methods Chemistry Copper Copper - chemistry Enzymes Exact sciences and technology Glucose Glucose - analysis Glucose Oxidase - metabolism Hydrogen Peroxide - analysis Imidazoles - chemistry Life Sciences Limit of Detection Luminescent Measurements - methods
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Zusammenfassung:	The effect of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO4]) on the copper-catalyzed luminol chemiluminescence (CL) is reported. A drastic light emission enhancement is observed, related to a strong interaction between Cu2+ and the imidazolium ring. In these conditions, the CL reaction was able to produce light efficiently at pH as low as 6.5 (amplification factor: Intensity+IL/Intensity−IL = 2900). Interesting effects of [Emim][EtSO4] on the enzyme glucose oxidase activity were also evidenced, and advantages were taken from this enhancement to perform sensitive chemiluminescent glucose detection (LOD = 4 μM) at pH 8.0.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac9026725