Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma
BackgroundIn conjunctival melanoma, little is known about the tumour biology and protein-expression patterns. In this study, the authors analysed the expression of the antiapoptotic oncoprotein B cell leukaemia/lymphoma-2 protein (Bcl-2), the tumour-suppressor phosphatase and tensin homologue delete...
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| Veröffentlicht in: | British journal of ophthalmology 2011-06, Vol.95 (6), p.853-858 |
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| creator | Westekemper, Henrike Karimi, Sara Süsskind, Daniela Anastassiou, Gerasimos Freistühler, Michael Steuhl, Klaus-Peter Bornfeld, Norbert Schmid, Kurt-Werner Grabellus, Florian |
| description | BackgroundIn conjunctival melanoma, little is known about the tumour biology and protein-expression patterns. In this study, the authors analysed the expression of the antiapoptotic oncoprotein B cell leukaemia/lymphoma-2 protein (Bcl-2), the tumour-suppressor phosphatase and tensin homologue deleted on chromosome ten (PTEN), and the heat-shock-protein HSP-90 in conjunctival melanoma (CoM) and conjunctival nevi (CoN) by immunohistochemistry (IHC).Material and methodsIHC was performed on 70 samples of CoM and 12 samples of CoN. Expression patterns between the diagnosis groups were compared. A receiver operating characteristic analysis was performed to determine the diagnostic value of the antigens.ResultsHSP-90 (p |
| doi_str_mv | 10.1136/bjo.2010.183939 |
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| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00595933v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>868376206</sourcerecordid><originalsourceid>FETCH-LOGICAL-b502t-effe139970bcdeb3a0b28751533050c4a48024a4029c8124cc11c9a5c8b861e03</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EotuWMzcUCaEKRNoZO_HHoYd2tWWRllJBQdwsx-uIhCTexpuq_Pc4yrJIXHoZa8a_eXr2I-Qlwiki42dF7U8pjJ1kiqknZIYZlykFoZ6SGQCIFJHjATkMoY4t5SiekwMKKudUwoycLx42vQuh8l3iy2T59SZR8D65uV1cJ6ZbJ5e2SWlSdYn1XT10dlvdmyZpXWM635pj8qw0TXAvducR-Xa1uJ0v09XnDx_nF6u0yIFuU1eWDplSAgq7dgUzUFApcswZgxxsZjIJNFagykqkmbWIVpncykJydMCOyNtJ96dp9KavWtP_1t5Uenmx0uMMIFe5YuweI3sysZve3w0ubHVbBeua6Nj5IWjJJROcAo_k6__I2g99Fx-iUQipOAMc9c4myvY-hN6VewMIegxBxxD0GIKeQogbr3a6Q9G69Z7_--sReLMDTLCmKXvT2Sr84zJKmUARuXTiqrB1D_t70__SXDCR6-vvc_1JXoovP8SVHq2-m_iirR91-QcwuKXC</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1778963011</pqid></control><display><type>article</type><title>Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma</title><source>MEDLINE</source><source>BMJ Journals - NESLi2</source><source>PubMed Central</source><creator>Westekemper, Henrike ; Karimi, Sara ; Süsskind, Daniela ; Anastassiou, Gerasimos ; Freistühler, Michael ; Steuhl, Klaus-Peter ; Bornfeld, Norbert ; Schmid, Kurt-Werner ; Grabellus, Florian</creator><creatorcontrib>Westekemper, Henrike ; Karimi, Sara ; Süsskind, Daniela ; Anastassiou, Gerasimos ; Freistühler, Michael ; Steuhl, Klaus-Peter ; Bornfeld, Norbert ; Schmid, Kurt-Werner ; Grabellus, Florian</creatorcontrib><description>BackgroundIn conjunctival melanoma, little is known about the tumour biology and protein-expression patterns. In this study, the authors analysed the expression of the antiapoptotic oncoprotein B cell leukaemia/lymphoma-2 protein (Bcl-2), the tumour-suppressor phosphatase and tensin homologue deleted on chromosome ten (PTEN), and the heat-shock-protein HSP-90 in conjunctival melanoma (CoM) and conjunctival nevi (CoN) by immunohistochemistry (IHC).Material and methodsIHC was performed on 70 samples of CoM and 12 samples of CoN. Expression patterns between the diagnosis groups were compared. A receiver operating characteristic analysis was performed to determine the diagnostic value of the antigens.ResultsHSP-90 (p<0.0001) and PTEN (p=0.001) showed the potential to differentiate between CoM and CoN. Bcl-2 expression was higher in CoM than in CoN (p=0.04). The loss of nuclear PTEN expression was more pronounced in the malignant melanomas than in CoN (p=0.02). Tumours located at unfavourable sites (fornix, palpebral conjunctiva, caruncle) that had developed recurrences expressed almost twice as much HSP-90 than recurrence-free tumours.ConclusionsConjunctival melanocytes differentially express Bcl-2, HSP-90 and PTEN, depending on their entity. HSP-90- and PTEN expression may add relevant information for the differentiation between conjunctival melanoma and nevi.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.2010.183939</identifier><identifier>PMID: 20956280</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>Antigens ; Bcl-2 ; Biological and medical sciences ; Chemotherapy ; conjunctiva ; Conjunctival Neoplasms - metabolism ; Dermatology ; Diagnosis, Differential ; Female ; HSP 90 ; HSP90 Heat-Shock Proteins - metabolism ; Humans ; Immunohistochemistry ; Male ; Medical prognosis ; Medical sciences ; melanocytes ; Melanoma ; Melanoma - metabolism ; Metastasis ; Middle Aged ; Miscellaneous ; Mutation ; neoplasia ; Neoplasm Proteins - metabolism ; Nevus, Pigmented - metabolism ; ocular surface ; Ophthalmology ; pathology ; Phosphatase ; Proteins ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; PTEN ; PTEN Phosphohydrolase - metabolism ; Reproducibility of Results ; Skin cancer ; Tumors ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>British journal of ophthalmology, 2011-06, Vol.95 (6), p.853-858</ispartof><rights>2011, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright: 2011 (c) 2011, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b502t-effe139970bcdeb3a0b28751533050c4a48024a4029c8124cc11c9a5c8b861e03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://bjo.bmj.com/content/95/6/853.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttp://bjo.bmj.com/content/95/6/853.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,230,314,776,780,881,3183,23550,27901,27902,77342,77373</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24223717$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20956280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00595933$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Westekemper, Henrike</creatorcontrib><creatorcontrib>Karimi, Sara</creatorcontrib><creatorcontrib>Süsskind, Daniela</creatorcontrib><creatorcontrib>Anastassiou, Gerasimos</creatorcontrib><creatorcontrib>Freistühler, Michael</creatorcontrib><creatorcontrib>Steuhl, Klaus-Peter</creatorcontrib><creatorcontrib>Bornfeld, Norbert</creatorcontrib><creatorcontrib>Schmid, Kurt-Werner</creatorcontrib><creatorcontrib>Grabellus, Florian</creatorcontrib><title>Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>BackgroundIn conjunctival melanoma, little is known about the tumour biology and protein-expression patterns. In this study, the authors analysed the expression of the antiapoptotic oncoprotein B cell leukaemia/lymphoma-2 protein (Bcl-2), the tumour-suppressor phosphatase and tensin homologue deleted on chromosome ten (PTEN), and the heat-shock-protein HSP-90 in conjunctival melanoma (CoM) and conjunctival nevi (CoN) by immunohistochemistry (IHC).Material and methodsIHC was performed on 70 samples of CoM and 12 samples of CoN. Expression patterns between the diagnosis groups were compared. A receiver operating characteristic analysis was performed to determine the diagnostic value of the antigens.ResultsHSP-90 (p<0.0001) and PTEN (p=0.001) showed the potential to differentiate between CoM and CoN. Bcl-2 expression was higher in CoM than in CoN (p=0.04). The loss of nuclear PTEN expression was more pronounced in the malignant melanomas than in CoN (p=0.02). Tumours located at unfavourable sites (fornix, palpebral conjunctiva, caruncle) that had developed recurrences expressed almost twice as much HSP-90 than recurrence-free tumours.ConclusionsConjunctival melanocytes differentially express Bcl-2, HSP-90 and PTEN, depending on their entity. HSP-90- and PTEN expression may add relevant information for the differentiation between conjunctival melanoma and nevi.</description><subject>Antigens</subject><subject>Bcl-2</subject><subject>Biological and medical sciences</subject><subject>Chemotherapy</subject><subject>conjunctiva</subject><subject>Conjunctival Neoplasms - metabolism</subject><subject>Dermatology</subject><subject>Diagnosis, Differential</subject><subject>Female</subject><subject>HSP 90</subject><subject>HSP90 Heat-Shock Proteins - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medical sciences</subject><subject>melanocytes</subject><subject>Melanoma</subject><subject>Melanoma - metabolism</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Mutation</subject><subject>neoplasia</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Nevus, Pigmented - metabolism</subject><subject>ocular surface</subject><subject>Ophthalmology</subject><subject>pathology</subject><subject>Phosphatase</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>PTEN</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>Reproducibility of Results</subject><subject>Skin cancer</subject><subject>Tumors</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1v1DAQxS0EotuWMzcUCaEKRNoZO_HHoYd2tWWRllJBQdwsx-uIhCTexpuq_Pc4yrJIXHoZa8a_eXr2I-Qlwiki42dF7U8pjJ1kiqknZIYZlykFoZ6SGQCIFJHjATkMoY4t5SiekwMKKudUwoycLx42vQuh8l3iy2T59SZR8D65uV1cJ6ZbJ5e2SWlSdYn1XT10dlvdmyZpXWM635pj8qw0TXAvducR-Xa1uJ0v09XnDx_nF6u0yIFuU1eWDplSAgq7dgUzUFApcswZgxxsZjIJNFagykqkmbWIVpncykJydMCOyNtJ96dp9KavWtP_1t5Uenmx0uMMIFe5YuweI3sysZve3w0ubHVbBeua6Nj5IWjJJROcAo_k6__I2g99Fx-iUQipOAMc9c4myvY-hN6VewMIegxBxxD0GIKeQogbr3a6Q9G69Z7_--sReLMDTLCmKXvT2Sr84zJKmUARuXTiqrB1D_t70__SXDCR6-vvc_1JXoovP8SVHq2-m_iirR91-QcwuKXC</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Westekemper, Henrike</creator><creator>Karimi, Sara</creator><creator>Süsskind, Daniela</creator><creator>Anastassiou, Gerasimos</creator><creator>Freistühler, Michael</creator><creator>Steuhl, Klaus-Peter</creator><creator>Bornfeld, Norbert</creator><creator>Schmid, Kurt-Werner</creator><creator>Grabellus, Florian</creator><general>BMJ Publishing Group Ltd</general><general>BMJ Publishing Group</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20110601</creationdate><title>Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma</title><author>Westekemper, Henrike ; Karimi, Sara ; Süsskind, Daniela ; Anastassiou, Gerasimos ; Freistühler, Michael ; Steuhl, Klaus-Peter ; Bornfeld, Norbert ; Schmid, Kurt-Werner ; Grabellus, Florian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b502t-effe139970bcdeb3a0b28751533050c4a48024a4029c8124cc11c9a5c8b861e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antigens</topic><topic>Bcl-2</topic><topic>Biological and medical sciences</topic><topic>Chemotherapy</topic><topic>conjunctiva</topic><topic>Conjunctival Neoplasms - metabolism</topic><topic>Dermatology</topic><topic>Diagnosis, Differential</topic><topic>Female</topic><topic>HSP 90</topic><topic>HSP90 Heat-Shock Proteins - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Medical sciences</topic><topic>melanocytes</topic><topic>Melanoma</topic><topic>Melanoma - metabolism</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Mutation</topic><topic>neoplasia</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Nevus, Pigmented - metabolism</topic><topic>ocular surface</topic><topic>Ophthalmology</topic><topic>pathology</topic><topic>Phosphatase</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>PTEN</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>Reproducibility of Results</topic><topic>Skin cancer</topic><topic>Tumors</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Westekemper, Henrike</creatorcontrib><creatorcontrib>Karimi, Sara</creatorcontrib><creatorcontrib>Süsskind, Daniela</creatorcontrib><creatorcontrib>Anastassiou, Gerasimos</creatorcontrib><creatorcontrib>Freistühler, Michael</creatorcontrib><creatorcontrib>Steuhl, Klaus-Peter</creatorcontrib><creatorcontrib>Bornfeld, Norbert</creatorcontrib><creatorcontrib>Schmid, Kurt-Werner</creatorcontrib><creatorcontrib>Grabellus, Florian</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Westekemper, Henrike</au><au>Karimi, Sara</au><au>Süsskind, Daniela</au><au>Anastassiou, Gerasimos</au><au>Freistühler, Michael</au><au>Steuhl, Klaus-Peter</au><au>Bornfeld, Norbert</au><au>Schmid, Kurt-Werner</au><au>Grabellus, Florian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>95</volume><issue>6</issue><spage>853</spage><epage>858</epage><pages>853-858</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>BackgroundIn conjunctival melanoma, little is known about the tumour biology and protein-expression patterns. In this study, the authors analysed the expression of the antiapoptotic oncoprotein B cell leukaemia/lymphoma-2 protein (Bcl-2), the tumour-suppressor phosphatase and tensin homologue deleted on chromosome ten (PTEN), and the heat-shock-protein HSP-90 in conjunctival melanoma (CoM) and conjunctival nevi (CoN) by immunohistochemistry (IHC).Material and methodsIHC was performed on 70 samples of CoM and 12 samples of CoN. Expression patterns between the diagnosis groups were compared. A receiver operating characteristic analysis was performed to determine the diagnostic value of the antigens.ResultsHSP-90 (p<0.0001) and PTEN (p=0.001) showed the potential to differentiate between CoM and CoN. Bcl-2 expression was higher in CoM than in CoN (p=0.04). The loss of nuclear PTEN expression was more pronounced in the malignant melanomas than in CoN (p=0.02). Tumours located at unfavourable sites (fornix, palpebral conjunctiva, caruncle) that had developed recurrences expressed almost twice as much HSP-90 than recurrence-free tumours.ConclusionsConjunctival melanocytes differentially express Bcl-2, HSP-90 and PTEN, depending on their entity. HSP-90- and PTEN expression may add relevant information for the differentiation between conjunctival melanoma and nevi.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>20956280</pmid><doi>10.1136/bjo.2010.183939</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Bcl-2 Biological and medical sciences Chemotherapy conjunctiva Conjunctival Neoplasms - metabolism Dermatology Diagnosis, Differential Female HSP 90 HSP90 Heat-Shock Proteins - metabolism Humans Immunohistochemistry Male Medical prognosis Medical sciences melanocytes Melanoma Melanoma - metabolism Metastasis Middle Aged Miscellaneous Mutation neoplasia Neoplasm Proteins - metabolism Nevus, Pigmented - metabolism ocular surface Ophthalmology pathology Phosphatase Proteins Proto-Oncogene Proteins c-bcl-2 - metabolism PTEN PTEN Phosphohydrolase - metabolism Reproducibility of Results Skin cancer Tumors Tumors of the skin and soft tissue. Premalignant lesions |
| title | Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma |
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