Straightforward Approach for Cellular‐Uptake Quantification

Straightforward Approach for Cellular‐Uptake Quantification

Putting a number on it: MALDI‐TOF MS enabled direct quantification of the cellular uptake of cell‐penetrating peptides (CPPs) by MDA‐MB‐231 breast cancer cells. This sensitive general strategy (see schematic representation), which requires no purification or separation steps, relies on the enhanceme...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2010-10, Vol.49 (44), p.8240-8243
Hauptverfasser: Paramelle, David, Subra, Gilles, Vezenkov, Lubomir L, Maynadier, Marie, André, Christophe, Enjalbal, Christine, Calmès, Monique, Garcia, Marcel, Martinez, Jean, Amblard, Muriel
Format: Artikel
Sprache:eng
Schlagworte:
cell-penetrating peptides
cellular uptake
Chemical Sciences
isotopic labeling
mass spectrometry
Organic chemistry
Peptides - metabolism
Protein Transport
quantification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Zusammenfassung:Putting a number on it: MALDI‐TOF MS enabled direct quantification of the cellular uptake of cell‐penetrating peptides (CPPs) by MDA‐MB‐231 breast cancer cells. This sensitive general strategy (see schematic representation), which requires no purification or separation steps, relies on the enhancement and discrimination of the MS signals of an α‐cyano‐4‐hydroxycinnamic acid tag in a neutral α‐cyano‐4‐hydroxycinnamic methyl ester matrix.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201003347