Development and use of real-time PCR to detect and quantify and “ Mycoplasma haematoparvum” in dogs

Two canine haemoplasma species have been recognised to date; (), which has been associated with anaemia in splenectomised or immunocompromised dogs, and “ Mycoplasma haematoparvum” (), recently described in an anaemic splenectomised dog undergoing chemotherapy. The study aim was to develop quantitative real-time PCR assays (qPCRs) incorporating an endogenous internal control to detect and and to apply these assays to DNA samples extracted from canine blood collected in Northern Tanzania (n=100) and from dogs presented to a Trinidadian veterinary hospital (n=185).