Live-Cell One- and Two-Photon Uncaging of a Far-Red Emitting Acridinone Fluorophore

Total synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which al...

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Veröffentlicht in:	Journal of the American Chemical Society 2010-03, Vol.132 (8), p.2585-2590
Hauptverfasser:	Warther, David, Bolze, Frédéric, Léonard, Jérémie, Gug, Sylvestre, Specht, Alexandre, Puliti, David, Sun, Xiao-Hua, Kessler, Pascal, Lutz, Yves, Vonesch, Jean-Luc, Winsor, Barbara, Nicoud, Jean-François, Goeldner, Maurice
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridines - analysis Acridines - chemical synthesis Acridines - metabolism Biochemistry, Molecular Biology Cell Membrane Permeability Cellular Biology Fluorescent Dyes - analysis Fluorescent Dyes - chemical synthesis Fluorescent Dyes - metabolism HeLa Cells Humans Life Sciences Microscopy, Fluorescence - methods Models, Molecular Photochemistry
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Zusammenfassung:	Total synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which allows an efficient (≥95%) and rapid (
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja9074562