A multiple primer pairs polymerase chain reaction for the detection of human genital papillomavirus types

We described a polymerase chain reaction (PCR) based on the simultaneous detection of multiple strains of papillomavirus in a single reaction tube. This PCR method was specific and sensitive. We have validated this multiplex procedure on a collection of typed cervical biopsies specimens, and applied...

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Veröffentlicht in:	Molecular and cellular probes 1993-10, Vol.7 (5), p.339-344
Hauptverfasser:	Lucotte, G., François, M.-H., Petit, M.-C., Berriche, S., Réveilleau, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Base Sequence Bioengineering Biological and medical sciences Cervical Intraepithelial Neoplasia Cervical Intraepithelial Neoplasia - diagnosis Cervix Uteri Cervix Uteri - microbiology DNA Primers DNA, Viral DNA, Viral - analysis Female Genital human papillomavirus (HPV), polymerase chain reaction (PCR), multiple primer pairs Human viral diseases Humans Infectious diseases Life Sciences Medical sciences Molecular Sequence Data Papillomaviridae Papillomaviridae - genetics Papillomaviridae - isolation & purification Papillomavirus Infections Papillomavirus Infections - diagnosis Polymerase Chain Reaction Polymerase Chain Reaction - methods Sensitivity and Specificity Tumor Virus Infections Tumor Virus Infections - diagnosis Uterine Cervical Diseases Uterine Cervical Diseases - diagnosis Vaginal Smears Viral diseases Viral diseases of the genital and urinary system
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Beschreibung
Zusammenfassung:	We described a polymerase chain reaction (PCR) based on the simultaneous detection of multiple strains of papillomavirus in a single reaction tube. This PCR method was specific and sensitive. We have validated this multiplex procedure on a collection of typed cervical biopsies specimens, and applied it to the detection of viruses in some clinical samples.
ISSN:	0890-8508 1096-1194
DOI:	10.1006/mcpr.1993.1050