Structure of an extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83

The extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83 was found to be composed of D-glucose and D-galactose in a molar ratio of 2:3. The primary structure of the polysaccharide was shown by sugar analysis, methylation analysis, FABMS, partial acid hydrolysis and nuclear mag...

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Veröffentlicht in:	Carbohydrate research 1998-12, Vol.314 (3-4), p.211-220
Hauptverfasser:	Vanhaverbeke, C, Bosso, C, Colin-Morel, P, Gey, C, Gamar-Nourani, L, Blondeau, K, Simonet, J M, Heyraud, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrate Sequence Gas Chromatography-Mass Spectrometry Lactobacillus - chemistry Magnetic Resonance Spectroscopy Methylation Molecular Sequence Data Molecular Structure Polysaccharides - chemistry Polysaccharides - isolation & purification
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Beschreibung
Zusammenfassung:	The extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83 was found to be composed of D-glucose and D-galactose in a molar ratio of 2:3. The primary structure of the polysaccharide was shown by sugar analysis, methylation analysis, FABMS, partial acid hydrolysis and nuclear magnetic resonance (NMR) spectroscopy to consist of a pentasaccharide repeating unit having the following structure: -->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->6)-alpha-D-Galp-(1-->6 )-alpha-D -Glcp-(1-->3)-beta-D-Galf-(1-->
ISSN:	0008-6215 0008-6215
DOI:	10.1016/S0008-6215(98)00297-3