RNAi-Mediated Allelic trans-Interaction at the Imprinted Rtl1/Peg11 Locus

The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally ex...

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Veröffentlicht in:	Current biology 2005-04, Vol.15 (8), p.743-749
Hauptverfasser:	Davis, Erica, Caiment, Florian, Tordoir, Xavier, Cavaillé, Jérôme, Ferguson-Smith, Anne, Cockett, Noelle, Georges, Michel, Charlier, Carole
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biochemistry, Molecular Biology Computational Biology Gene Components Genomic Imprinting - genetics Glycoproteins - genetics Inheritance Patterns - genetics Life Sciences Mammals - genetics MicroRNAs - genetics MicroRNAs - metabolism Molecular Sequence Data Mutation - genetics Proteins - genetics Proteins - metabolism RNA Interference RNA, Messenger - metabolism Sequence Alignment
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Beschreibung
Zusammenfassung:	The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally expressed intronless gene with homology to the gag and pol polyproteins of Sushi-like retroelements [3]. We herein demonstrate that several additional miRNAs are processed from antiPeg11 and that these regulate Rtl1/Peg11 in trans by guiding RISC-mediated cleavage of its mRNA. This is the first demonstration of miRNA-mediated RNAi involving imprinted genes in mammals.
ISSN:	0960-9822 1879-0445
DOI:	10.1016/j.cub.2005.02.060