The effect of polyherbal containing Tamarindus indica and Murraya paniculata leaf extract on triglyceride level using a rat model of hypertriglyceridemia

The current anti-hypertriglyceridemia (HTG) medications still have side effects such as myopathy, rhabdomyolysis, liver damage, and insulin resistance. The development and discovery of new, safer agents to treat hypertriglyceridemia is urgently needed. The leaves of Murraya paniculata have been empirically used for lowering lipids. Meanwhile, Tamarindus indica leaves are reported to contain high antioxidants and anti-diabetic activity. This study was conducted to evaluate the combination effect of leaf extracts from Tamarindus indica and Murraya paniculata to treat hypertriglyceridemia in animal models and to predict the optimum ratio. Hypertriglyceridemic Wistar rats were developed by feeding the high fat-sucrose diet for 75 days. The combination of T. indica extract (TIE) and M. paniculata extract (MPE) was made following the Simplex Lattice Design ranging from 50:350 to 350:50, producing denoted ratios TIE:MPE: (i) 50:350, (ii) 125:275, (iii) 200:200, (iv) 275:125, and (v) 350:50. The extract was given to hyperlipidemic animals orally once per day for 14 days at a dose of 400 mg/kgBW. Prior to the in vivo assay, both extracts were analyzed for their phytochemical content (mineral, phenol and flavonoid content) and water content. The result of lipid profile measurements showed that the combination of 350:50 mg/kgBW (TIE:MPE) gave the strongest effect in lowering triglyceride (p