Protective Effect of Polysaccharides Isolated from Sargassum horneri against H[sub.2]O[sub.2]-Induced Oxidative Stress Both In Vitro, in Vero Cells, and In Vivo in Zebrafish

The environment within which human beings exist is progressively becoming more complex. A multitude of external factors prompt the body to produce excessive amounts of reactive oxygen species (ROS), thereby leading to heightened levels of oxidative stress, which is detrimental to health. In recent years, researchers have discerned the potential of secondary metabolites derived from brown algae in combating diseases. Additionally, Sargassum horneri has witnessed a large-scale outbreak in China, exerting a severe negative influence on the local ecology and economic development. We extracted polysaccharides (SHPs) from S. horneri and discovered that SHPs possess outstanding antioxidant properties and can be utilized as a natural antioxidant. Our work can alleviate the environmental stress caused by algae and achieve the utilization of algae resources, which hold considerable social value and significance. The extensive outbreak of Sargassum horneri in China has not merely imposed a severe threat to the ecological environment and human life in coastal waters but also impeded the development of waterway transportation and the local economy. Consequently, we isolated polysaccharides from S. horneri, designated as SHP, and evaluated the antioxidant activity of SHP both in vitro and in vivo by investigating the effect of SHP on H[sub.2]O[sub.2]-induced African green monkey kidney cells (Vero cells) and zebrafish. The results demonstrated that SHP can enhance the activities of superoxide dismutase, catalase, and glutathione peroxidase in zebrafish. It also effectively inhibits micro malondialdehyde and ROS levels in Vero cells and zebrafish to mitigate the oxidative damage caused by H[sub.2]O[sub.2], thereby achieving the protective effect of SHP on Vero cells and zebrafish. In conclusion, SHP holds the potential as a natural antioxidant. SHP can be contemplated for utilization as a natural antioxidant in the biomedical, cosmetic, and food industries, thereby alleviating the environmental stress caused by S. horneri and achieving resource utilization.