Negative effects of ethanol on ovarian reserve and endometrium thickness: An animal study/Etanolun yumurtalik rezervi ve endometrium kalinligi uzerine olumsuz etkileri: Bir hayvan calismasi
Objective: This study aimed to assess the effect of ethanol on the ovarian reserve and endometrium of rats by evaluating anti-Mullerian hormone (AMH) levels and follicle counts. Materials and Methods: We performed histological follicle counting and AMH measurements to evaluate ovarian reserve. The s...
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Veröffentlicht in: | Turkish journal of obstetrics and gynecology 2024-09, Vol.29 (3), p.175 |
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Sprache: | eng |
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Zusammenfassung: | Objective: This study aimed to assess the effect of ethanol on the ovarian reserve and endometrium of rats by evaluating anti-Mullerian hormone (AMH) levels and follicle counts. Materials and Methods: We performed histological follicle counting and AMH measurements to evaluate ovarian reserve. The study included 16 Wistar albino rats evenly distributed into two groups of eight rats each. The rats in the intervention group (group 1) were administered ethanol at a daily dose of 2.5 g/kg via oral gastric lavage for 30 days, whereas the control group (group 2) received water as a placebo via oral gastric lavage for the same period. At the end of 30 days, the animals were sacrificed, and 2 mL blood samples were collected for AMH measurements. Laparotomy was performed to remove the ovaries and uterus. Results: Despite the lack of a meaningful distinction in the quantity of primordial and primary follicles between the two groups, a substantial disparity was observed in the overall follicle count and AMH levels. Specifically, the intervention group exhibited significantly lower total follicle counts and AMH levels than the control group (p[less than or equal to]0.001). The researchers also found that the endometrium of ethanol-treated rats was significantly thinner than that of control rats (p[less than or equal to]0.001). Conclusion: This study concluded that ethanol consumption can negatively affect reproductive ability and the success of in vitro fertilization treatment by reducing ovarian reserve and thinning the endometrium. Keywords: Ethanol, endometrium, ovarian reserve, rat Ama : Bu alismada, anti-M llerian hormon (AMH) d zeyleri ve folik l sayilari degerlendirilerek etanol n si anlarin ovaryan rezervi ve endometriyumu zerindeki etkisinin degerlendirilmesi ama lanmistir. Gere ve Y ntemler: Arastirmacilar ovaryum rezervini degerlendirmek i in histolojik folik l sayimi ve AMH l mleri ger eklestirmistir. alismaya her biri sekiz si andan olusan iki gruba esit olarak dagitilmis 16 Wistar albino si an dahil edilmistir. M dahale grubundaki si anlara (grup 1) 30 g n boyunca oral gastrik lavaj yoluyla g nl k 2,5 g/kg dozunda etanol uygulanirken, kontrol grubuna (grup 2) ayni s re boyunca oral gastrik lavaj yoluyla plasebo olarak su verilmistir. Otuz g n n sonunda hayvanlar sakrifiye edilmis ve AMH l m i in 2 mL kan rnegi alinmistir. Yumurtaliklari ve uterusu ikarmak i in laparotomi yapildi. Bulgular: Iki grup arasinda primordial ve primer folik l miktarinda anlamli |
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ISSN: | 2149-9322 |
DOI: | 10.4274/tjod.galenos.2024.89439 |