In vitro Biomedical Application of Endophytic Aspergillus me melleus Isolated from Leaves of Premna serratifolia L

Some microorganisms known as endophytes live in symbiotic relationships in the living tissues of plants without posing a health risk. As a result, they synthesize many metabolites which are helpful for the plants in many ways. So, these metabolites are known to exhibit many biological properties like antioxidant, antidiabetic anti-inflammatory, etc. Currently, many drugs are used to control inflammatory diseases like arthritis and, irritable bowel disease; however, they pose a lot of side effects. The present study was taken up to explore the anti-inflammatory properties along with the phytochemicals present, its quantification, and other in vitro biological activities of the less reported Aspergillus melleus, an endophytic fungus, isolated from Premna serratifolia L, a medicinal plant. The results of the investigation demonstrated the presence of alkaloids, phenols, terpenoids, flavonoids, tannins, cardiac glycosides and amino acids in the methanolic extract of endophytic fungus. It yielded 25.28 [micro]g GAE/g and 19.465 [micro]g GAE/g of total phenolic and flavonoid content, respectively. The results of anti-inflammatory activity showed 84.69+0.82% protein inhibition by BSA and also showed [IC.sub.50] values of 68.53 [micro]g/mL and 43.34 [micro]g/mL for COX1 and COX 2, respectively. It exhibited 63.91[+ or -]0.08% of radical scavenging activity by DPPH. The [IC.sub.50] values of 181.41 [micro]g/mL and 190.62 [micro]g/mL were found for the in vitro antidiabetic activity. This study shows that the endophytic fungus A. melleus has exhibited considerably good results with respect to its in vitro biological activities. Yet, there is a scope for future researchers to isolate the bioactive metabolites to explore for future needs. Key words: Aspergillus melleus, Anti-inflammatory Activity, COX-1, COX-2, Antidiabetic Activity