In vivo Gonadoprotective Effects of Myricetin on Cisplatin-Induced Testicular Damage via Suppression of TLR4/NF-[kappa]B Inflammation Pathway and Heat-Shock Response/Efectos Gonadoprotectores in vivo de la Miricetina sobre el Dano Testicular Inducido por Cisplatino Mediante la Supresion de la Via de Inflamacion TLR4/NF-[kappa]B y la Respuesta al Choque Termico

The aim of this study is to reveal the gonadoprotective effects of myricetin (MYC), which has many biological properties, on cisplatin (CP)-induced testicular damage in rats. For this purpose, 40 male Wistar albino rats were divided into 4 groups as Control (group given no treatment), MYC (group given 5 mg/kg/i.p myricetin for 7 days), CP (group given 7 mg/kg/i.p cisplatin at 7th day) and MYC + CP (group given 5 mg/kg/i.p myricetin for 7 days before 7 mg/kg/i.p cisplatin injection). After administrations, testicular tissues of animals were extracted and processed according to tissue processing protocol. Hematoxylin & Eosin staining were performed to evaluate the histopathological changes and Johnsen'sTesticular Biopsy Score (JTBS) was applied and mean seminiferous tubule diameters (MSTD) were measured to compare experimental groups in terms of histopathological changes. Moreover, TLR4, NF-[kappa]B, HSP70 and HSP90 expression levels were detected by immunohistochemical staining and the density of immunoreactivity were measured to determine the difference in the expression levels of these factors among groups. Additionally, testicular apoptosis was detected via TUNEL assay. JTBS and MSTD data were significantly lower in CP group compared to other groups and MYC administrations significantly protects testicular tissue against CP-induced damage. Moreover, TLR4, NF-[kappa]B, HSP70 and HSP90 expressions and apoptotic cells significantly increased in the CP group (p