Determination of the antimicrobial activity of lactic acid bacteria isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819

The present study reports on the determination of the antimicrobial activity of lactic acid bacteria (LAB) isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819. The samples were collected in the period of August 2018 until March 2021. The BIOLOG system was used for microbiological determination. From the mussel M. galloprovincialis Lam. four species of LAB were isolated - Sporolactobacillus kofuensis , Lactobacillus sakei , Streptococcus gallolyticus ss gallolyticus and Lactibacillus brevis . The activity of the strains was determined against test cultures ( Escherichia coli 3398 , Staphylococus aureus 745 , Bacillus subtilis 6633 , Salmonella typhimurium 3591 , Listeria monocytogens 863 Enterobacter aerogenes 3691 , Aspergillus niger , Penicillium claviforme , Saccharomyces cerevisae , Candida albicans 8673 and Candida glabrata 72 ). Before the analysis for antimicrobial activity, the LAB were cultured in media with different concentrations of sugars - 2, 5 and 10%. The results showed that 4 strains S. kofuensis , L. sakei , S. gallolyticus ss gallolyticus and L. brevis cultured on glucose and oligosaccharides completely lost their activity in all studied variants. Therefore, some carbohydrates (glucose) and oligosaccharides induce the synthesis outside the cell of biologically active molecules, which can probably be attributed to peptides/proteins.