In Vitro Evaluation of Anti-Angiogenic Efficacy, Cell Migration Activity and Cell Survival Effect Against Hydrogen Peroxide-Induced Damage of Prunetin

Anti-angiogenic isoflavonoids are gaining attention as a novel approach in the prevention and treatment of tumor progression and metastasis. These phenylpropanoid compounds as bioactive phytoestrogens exhibit various pharmacological activities including antimicrobial, anti-inflammatory and antioxidant effects. The purpose of this study was to evaluate the anti-angiogenic, antioxidant and cell migration effects of prunetin in in vitro studies. The antioxidant activity of prunetin was performed against H 2 O 2 -induced cell damage on Vero cells. The cell migration effect was comparatively studied with HaCat cells. The anti-angiogenic potential of prunetin was assessed using an in vitro HET-CAM model. The strongest anti-angiogenic effect of prunetin was observed at 100 and 200 μM on the chorioallantoic membranes of hen eggs when compared to the control group. Similarly, the statistical analysis of data showed that prunetin treatment at 200 μM significantly induced cell migration with a ratio of 98.65 ± 0.46% on HaCaT cells. Additionally, the excessive protective effect of prunetin against H 2 O 2 -induced cytotoxicity in Vero cells was shown with 90% cell viability at the highest concentration. In conclusion, prunetin can stimulate keratinocytes migration and can induce antioxidant mechanisms which involves direct inhibition of the ROS generation and anti-angiogenic responses. It is suggested that prunetin can be applied to support anti-cancer treatment given its promising results since isoflavonoids possess antioxidant, anti-inflammatory and cell migration properties.