IRhodoalgimonas zhirmunskyi/I gen. nov., sp. nov., a Marine Alphaproteobacterium Isolated from the Pacific Red Alga IAhnfeltia tobuchiensis/I: Phenotypic Characterization and Pan-Genome Analysis

A novel Gram-staining negative, strictly aerobic, rod-shaped, and non-motile bacterium, designated strain 10Alg 79[sup.T] , was isolated from the red alga Ahnfeltia tobuchiensis. A phylogenetic analysis based on 16S rRNA gene sequences placed the novel strain within the family Roseobacteraceae, class Alphaproteobacteria, phylum Pseudomonadota, where the nearest neighbor was Shimia sediminis ZQ172[sup.T] (97.33% of identity). However, a phylogenomic study clearly showed that strain 10Alg 79[sup.T] forms a distinct evolutionary lineage at the genus level within the family Roseobacteraceae combining with strains Aquicoccus porphyridii L1 8-17[sup.T] , Marimonas arenosa KCTC 52189[sup.T] , and Lentibacter algarum DSM 24677[sup.T] . The ANI, AAI, and dDDH values between them were 75.63–78.15%, 67.41–73.08%, and 18.8–19.8%, respectively. The genome comprises 3,754,741 bp with a DNA GC content of 62.1 mol%. The prevalent fatty acids of strain 10Alg 79[sup.T] were C[sub.18:1 ω7c] and C[sub.16:0] . The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. A pan-genome analysis showed that the unique part of the 10Alg 79[sup.T] genome consists of 13 genus-specific clusters and 413 singletons. The annotated singletons were more often related to transport protein systems, transcriptional regulators, and enzymes. A functional annotation of the draft genome sequence revealed that this bacterium could be a source of a new phosphorylase, which may be used for phosphoglycoside synthesis. A combination of the genotypic and phenotypic data showed that the bacterial isolate represents a novel species and a novel genus, for which the name Rhodoalgimonas zhirmunskyi gen. nov., sp. nov. is proposed. The type strain is 10Alg 79[sup.T] (=KCTC 72611[sup.T] = KMM 6723[sup.T] ).