Development of quantification technique for multiconstituent phytoformulation with recap of effects of combination therapy

Background and Aims: The objective of this study was to extensively review therapeutic effects of co-administration of flavonoid curcuminoids and alkaloid piperine. Furthermore, the aim of this study was to develop a simple isocratic reversed-phase HPLC (RP-HPLC) method to quantify curcuminoids and piperine in the combined dosage form developed in-house. Methods: The RP-HPLC quantification was performed on Inersil ODS-3V, 150 mm x 4.6 mm, 5 [micro]m column using Acetonitrile: Buffer (35: 65% v/v) as a mobile phase, at a flow rate of 1.5 mL/min. The curcuminoids and piperine were detected at wavelengths of 420 nm and 342nm, respectively. The method was validated according to the International Council for Harmonization (ICH) guideline Q2(R1). Results: The individual curcuminoids and piperine peaks had theoretical plate number (N) > 4000 and a tailing factor (T) < 1.5 confirming well separation of the compounds. The calibration curve was linear from 0.6-18 [micro]g/mL and 0.2-6 [micro]g/mL for curcuminoids and piperine, respectively, with the correlation coefficient of >0.9990. The recovery and precision study values were in close agreement. The method was robust with relative standard deviation (RSD) less than 2%. Conclusion: The literature survey indicated that the co-administration of piperine had influenced pharmacodynamic and pharmacokinetic activities of curcuminoids. The analytical method developed was found to be specific, sensitive, precise, and accurate for the estimation of curcuminoids and piperine in a single run. Keywords: Co-administration, curcuminoids, piperine, RP-HPLC, validation