Effects of allium cepa on ovarian torsion-detorsion injury in a rat model/Ratlarda ovaryan torsiyon-detorsiyon modelinde allium cepanin etkilerinin incelenmesi

Objective: Ischemia/reperfusion (I/R) damage following detorsion treatment, tissue fibrosis, and adhesions cause secondary tissue damage in the ovaries. Many studies have been evaluated to minimize antioxidant damage in ovarian reserve loss while minimizing I/R damage. However, no study observed long-term effects on the ovarian torsion model in rats. In this study, we evaluated the profibrotic effects of A. cepa on an ovarian torsion model on rats. Materials and Methods: Group I (n=7) rats were the sham group. Group II (n=7) rats were the torsion group and Group III (n=7) rats were the torsion + A. cepa group. To observe the long-term effects of allium cepa, rats were fed for 21 days. Cellular damage I/R is evaluated by histopathological damage score, and transforming growth factor-beta 1 (TGF-[beta]1) and alpha-smooth muscle actin ([alpha]-SMA) is measured to analyze the profibrotic effect. Results: A. cepa altered cellular damage due to improvement in the histopathological damage score with A. cepa intake. However, the profibrotic mediators TGF-[beta]1 and a-SMA are non- significantly changed by the A. cepa (p=0.477 and p=0.185 respectively). Conclusion: A. cepa is a potent protective on cellular tissue, minimizing I/R damage on ovarian tissue histologically. Our study implies that A. cepa does not affect fibrosis-related mediators in the rat ovary. Keywords: Allium cepa, fibrosis, ovarian torsion, ischemia-reperfusion injury, TGF-[beta]1, a-SMA Amac: Over torsiyonunda tedavi amacli uygulanan detorsiyon islemine sekonder olusan iskemi/reperfuzyon hasarinin (I/R); dokuda olusan fibrozis ve adezyonlara bagli olarak over dokusuna sekonder hasar verebilir. Over torsiyonunda literaturde bircok calisma oxidan hasara bagli over rezervini minimalize etmeye yonelik bircok calisma mevcuttur. Ancak, literaturde iskemi hasarinin torsiyon sonrasi uzun donemde ovarian yapisal degisikliklerine dair bir calisma mevcut degildir. Bu calismada A. cepa'nin profibrotik mediatorler uzerine etkisinin incelenmesi rat modeli uzerinde amaclanmistir. Gerec ve Yontemler: Grup I (n=7) ratlar kontrol grubu olarak belirlenmistir. Grup II (n=7) torsiyon-detorsiyon modeli ve Grup III (n=7) torsion-detorsiyon + A. cepa rejimi uygulanacak gruplar olarak belirlenmistir. Adezyon ve fibrotik degisimlerin izlenmesi icin ratlar prosedur sonrasi 21 gun beslendi. Hucresel hasar duzeyi "Histopatolojik Hasar skoru" ile olculdu. Fibrotik degisiklikler icin donusen buyume faktoru-beta 1 (TGF-[beta]1