Dietary Fermentation Product of IAspergillus Oryzae/I Prevents Increases in Gastrointestinal Permeability in Horses Undergoing Combined Transport and Exercise

Equine leaky gut syndrome is characterized by gastrointestinal hyperpermeability and may be associated with adverse health effects in horses. The purpose was to evaluate the effects of a prebiotic Aspergillus oryzae product (SUPP) on the stress-induced leakiness of the gut. For 28 days, 8 horses received a diet containing the prebiotic or an unsupplemented diet (CO). On Days 0 and 28, horses were dosed with a compound (iohexol) that should only leak out of the gastrointestinal tract if the gut walls become leaky. Immediately following iohexol administration, four horses from each feeding group underwent 60 min of transport immediately followed by a moderate-intensity exercise bout of 30 min (EX), and the remaining horses were maintained as sedentary controls (SED). Blood was sampled before iohexol, immediately after trailering, and at 0, 1, 2, 4, and 8 h post-exercise. Blood was analyzed for iohexol, as well as lipopolysaccharide (a compound found in the gastrointestinal tract that can leak out) and serum amyloid A (a marker of inflammatory response). EX resulted in a significant increase in plasma iohexol in both CO and SUPP groups on Day 0; this increase was not seen in SED horses. On Day 28, EX increased plasma iohexol only in the CO feeding group; this increase was completely prevented by the provision of SUPP. It is concluded that combined transport and exercise induce leaky gut. Dietary SUPP prevents this and therefore may be a useful prophylactic for pathologies associated with gastrointestinal hyperpermeability in horses. Equine leaky gut syndrome is characterized by gastrointestinal hyperpermeability and may be associated with adverse health effects in horses. The purpose was to evaluate the effects of a prebiotic Aspergillus oryzae product (SUPP) on stress-induced gastrointestinal hyperpermeability. Eight horses received a diet containing SUPP (0.02 g/kg BW) or an unsupplemented diet (CO) (n = 4 per group) for 28 days. On Days 0 and 28, horses were intubated with an indigestible marker of gastrointestinal permeability (iohexol). Half the horses from each feeding group underwent 60 min of transport by trailer immediately followed by a moderate-intensity exercise bout of 30 min (EX), and the remaining horses stayed in stalls as controls (SED). Blood was sampled before iohexol, immediately after trailering, and at 0, 1, 2, 4, and 8 h post-exercise. At the end of the feeding period, horses were washed out for 28 days before being assigned to the oppo