Construction and Comprehensive Analysis of miRNAs and Target mRNAs in ILongissimus dorsi/I Muscle of Queshan Black and Large White Pigs

In this study, the miRNA-mRNA combination analysis of the longissimus dorsi of adult Queshan Black and Large White pigs was carried out by RNA-seq technology, and the molecular mechanism affecting pork quality traits was revealed. The results showed that 39 miRNAs were differentially expressed between Queshan Black and Large White pigs. A functional enrichment analysis of their target genes showed that they were mainly enriched in pathways related to adipogenesis and cell proliferation and differentiation. In addition, miR-328, as a key miRNA, can affect the proliferation of 3T3-L1 cells. This study revealed the miRNA-mRNA regulatory axis affecting fat deposition and skeletal muscle mass and provided a theoretical basis for further study of the molecular regulation mechanism of meat quality traits.